EXOSC9 Recombinant Rabbit Monoclonal Antibody [PSH08-55]
Catalog# HA723001
EXOSC9 Recombinant Rabbit Monoclonal Antibody [PSH08-55]
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
EXOSC9 Recombinant Rabbit Monoclonal Antibody [PSH08-55]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human EXOSC9 aa 1-350.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 49 kDa
Positive Control
293T cell lysate, HeLa cell lysate, HepG2 cell lysate, K-562 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, C6 cell lysate.
Conjugation
unconjugated
Clone Number
PSH08-55
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| WB | IHC-P | FC | IF-Cell | |
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| human |
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| mouse |
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| rat |
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Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000
Target
Function
Exosome component 9, also known as EXOSC9, is a human gene, the protein product of which (sometimes called RRP45 or PM/Scl-75) is part of the exosome complex and is an autoantigen is patients with certain auto immune diseases, most notably scleromyositis.
Background References
1. Yoshino S et al. EXOSC9 depletion attenuates P-body formation, stress resistance, and tumorigenicity of cancer cells. Sci Rep. 2020 Jun
2. Quttina M et al. Exosc9 Initiates SUMO-Dependent lncRNA TERRA Degradation to Impact Telomeric Integrity in Endocrine Therapy Insensitive Hormone Receptor-Positive Breast Cancer. Cells. 2023 Oct
Subcellular Location
Cytoplasm, Nucleus, nucleolus, nucleoplasm.
Synonyms
Autoantigen PM/Scl 1 antibody
EXOS9_HUMAN antibody
EXOSC 9 antibody
EXOSC9 antibody
Exosome complex component RRP45 antibody
Exosome complex exonuclease RRP45 antibody
Exosome component 9 antibody
p5 antibody
p6 antibody
P75 polymyositis scleroderma overlap syndrome associated autoantigen antibody
ExpandImages
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Western blot analysis of EXOSC9 on different lysates with Rabbit anti-EXOSC9 antibody (HA723001) at 1/2,000 dilution.
Lane 1: 293T cell lysate
Lane 2: HeLa cell lysate
Lane 3: HepG2 cell lysate
Lane 4: K-562 cell lysate
Lane 5: NIH/3T3 cell lysate
Lane 6: C2C12 cell lysate
Lane 7: C6 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 49 kDa
Observed band size: 70 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723001) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"