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TREX1 Recombinant Rabbit Monoclonal Antibody [PSH08-84] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751249

TREX1 Recombinant Rabbit Monoclonal Antibody [PSH08-84] - BSA and Azide free

Application

  • WB

  • FC

  • IP

Reactivity

  • Mouse

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

TREX1 Recombinant Rabbit Monoclonal Antibody [PSH08-84] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within mouse TREX1 aa 1-280.

Species Reactivity

Mouse

Validated Applications

WB, FC, IP

Molecular Weight

Predicted band size: 34 kDa

Positive Control

RAW264.7 cell lysate, CTLL-2 cell lysate, J774A.1 cell lysate, RAW264.7.

Conjugation

unconjugated

Clone Number

PSH08-84

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000-1:5,000

  • FC

  • 1:1,000

  • IP

  • 1-2μg/sample

Target

Function

Three prime repair exonuclease 1 is an enzyme that in humans is encoded by the TREX1 gene. This gene encodes the major 3'->5' DNA exonuclease in human cells. The protein is a non-processive exonuclease that may serve a proofreading function for a human DNA polymerase. It is also a component of the SET complex, and acts to rapidly degrade 3' ends of nicked DNA during granzyme A-mediated cell death. Mutations in this gene result in Aicardi-Goutieres syndrome, chilblain lupus, RVCL (Retinal Vasculopathy with Cerebral Leukodystrophy), and Cree encephalitis. Multiple transcript variants encoding different isoforms have been found for this gene.

Background References

1. Tani T et al. TREX1 Inactivation Unleashes Cancer Cell STING-Interferon Signaling and Promotes Antitumor Immunity. Cancer Discov. 2024 May

2. Lim J et al. The Exonuclease TREX1 Constitutes an Innate Immune Checkpoint Limiting cGAS/STING-Mediated Antitumor Immunity. Cancer Immunol Res. 2024 Jun

Subcellular Location

Nucleus, Cytoplasm, cytosol, Endoplasmic reticulum membrane.

UNIPROT

SwissProt: Q91XB0 Mouse

Synonyms

3' 5' exonuclease TREX1 antibody

3' repair exonuclease 1 antibody

AGS1 antibody

AGS5 antibody

CRV antibody

Deoxyribonuclease III, dnaQ/mutD (E. coli) like antibody

DKFZp434J0310 antibody

DNase III antibody

DRN3 antibody

HERNS antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of TREX1 on different lysates with Rabbit anti-TREX1 antibody (<a href="/products/HA751249" style="font-weight: bold;text-decoration: underline;">HA751249</a>) at 1/5,000 dilution.<br /><br />Lane 1: RAW264.7 cell lysate<br />Lane 2: CTLL-2 cell lysate<br />Lane 3: J774A.1 cell lysate<br />Lane 4: Neuro-2a cell lysate (negative)<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 34 kDa<br />Observed band size: 35 kDa<br /><br />Exposure time: Lane 1: 25 seconds; Lane 2-4: 1 minute; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751249" style="font-weight: bold;text-decoration: underline;">HA751249</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of TREX1 on different lysates with Rabbit anti-TREX1 antibody (HA751249) at 1/5,000 dilution.

    Lane 1: RAW264.7 cell lysate
    Lane 2: CTLL-2 cell lysate
    Lane 3: J774A.1 cell lysate
    Lane 4: Neuro-2a cell lysate (negative)

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 34 kDa
    Observed band size: 35 kDa

    Exposure time: Lane 1: 25 seconds; Lane 2-4: 1 minute; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751249) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Flow cytometric analysis of Neuro-2a (left, negative) and RAW264.7 (right, positive) cells labeling TREX1.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA751249" style="font-weight: bold;text-decoration: underline;">HA751249</a>, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    ☑ Relative expression (RE)

    Flow cytometric analysis of Neuro-2a (left, negative) and RAW264.7 (right, positive) cells labeling TREX1.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA751249, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

  • TREX1 was immunoprecipitated from 0.2 mg RAW264.7 cell lysate with <a href="/products/HA751249" style="font-weight: bold;text-decoration: underline;">HA751249</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA751249" style="font-weight: bold;text-decoration: underline;">HA751249</a> at 1/1,000 dilution. Mouse anti Rabbit IgG heavy chain (Fc) secondary antibody (<a href="/products/M1003-7" style="font-weight: bold;text-decoration: underline;">M1003-7</a>) at 1/10,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: RAW264.7 cell lysate (input)<br />Lane 2: <a href="/products/HA751249" style="font-weight: bold;text-decoration: underline;">HA751249</a> IP in RAW264.7 cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA751249" style="font-weight: bold;text-decoration: underline;">HA751249</a> in RAW264.7 cell lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 16 seconds; ECL: <a href="/products/K1801" style="font-weight: bold;text-decoration: underline;">K1801</a>

    TREX1 was immunoprecipitated from 0.2 mg RAW264.7 cell lysate with HA751249 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751249 at 1/1,000 dilution. Mouse anti Rabbit IgG heavy chain (Fc) secondary antibody (M1003-7) at 1/10,000 dilution was used for 1 hour at room temperature.

    Lane 1: RAW264.7 cell lysate (input)
    Lane 2: HA751249 IP in RAW264.7 cell lysate
    Lane 3: Rabbit IgG instead of HA751249 in RAW264.7 cell lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 16 seconds; ECL: K1801

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