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TREX1 Recombinant Rabbit Monoclonal Antibody [PSH08-84]

Usd: 385

Specification

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Catalog# HA723036

TREX1 Recombinant Rabbit Monoclonal Antibody [PSH08-84]

Application

  • WB

  • FC

  • IP

Reactivity

  • Mouse

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

TREX1 Recombinant Rabbit Monoclonal Antibody [PSH08-84]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within mouse TREX1 aa 1-280.

Species Reactivity

Mouse

Validated Applications

WB, FC, IP

Molecular Weight

Predicted band size: 34 kDa

Positive Control

RAW264.7 cell lysate, CTLL-2 cell lysate, J774A.1 cell lysate, RAW264.7.

Conjugation

unconjugated

Clone Number

PSH08-84

Reactivity Data

WB FC IP
Mouse
Tested Tested
Tested Tested
Tested Tested
Human
Not Recommended
Not Recommended
Rat
Not Recommended
Not Recommended

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000-1:5,000

  • FC

  • 1:1,000

  • IP

  • 1-2μg/sample

Target

Function

Three prime repair exonuclease 1 is an enzyme that in humans is encoded by the TREX1 gene. This gene encodes the major 3'->5' DNA exonuclease in human cells. The protein is a non-processive exonuclease that may serve a proofreading function for a human DNA polymerase. It is also a component of the SET complex, and acts to rapidly degrade 3' ends of nicked DNA during granzyme A-mediated cell death. Mutations in this gene result in Aicardi-Goutieres syndrome, chilblain lupus, RVCL (Retinal Vasculopathy with Cerebral Leukodystrophy), and Cree encephalitis. Multiple transcript variants encoding different isoforms have been found for this gene.

Background References

1. Tani T et al. TREX1 Inactivation Unleashes Cancer Cell STING-Interferon Signaling and Promotes Antitumor Immunity. Cancer Discov. 2024 May

2. Lim J et al. The Exonuclease TREX1 Constitutes an Innate Immune Checkpoint Limiting cGAS/STING-Mediated Antitumor Immunity. Cancer Immunol Res. 2024 Jun

Subcellular Location

Nucleus, Cytoplasm, cytosol, Endoplasmic reticulum membrane.

UNIPROT

SwissProt: Q91XB0 Mouse

Synonyms

3' 5' exonuclease TREX1 antibody

3' repair exonuclease 1 antibody

AGS1 antibody

AGS5 antibody

CRV antibody

Deoxyribonuclease III, dnaQ/mutD (E. coli) like antibody

DKFZp434J0310 antibody

DNase III antibody

DRN3 antibody

HERNS antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of TREX1 on different lysates with Rabbit anti-TREX1 antibody (<a href="/products/HA723036" style="font-weight: bold;text-decoration: underline;">HA723036</a>) at 1/5,000 dilution.<br /><br />Lane 1: RAW264.7 cell lysate<br />Lane 2: CTLL-2 cell lysate<br />Lane 3: J774A.1 cell lysate<br />Lane 4: Neuro-2a cell lysate (negative)<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 34 kDa<br />Observed band size: 35 kDa<br /><br />Exposure time: Lane 1: 25 seconds; Lane 2-4: 1 minute; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723036" style="font-weight: bold;text-decoration: underline;">HA723036</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of TREX1 on different lysates with Rabbit anti-TREX1 antibody (HA723036) at 1/5,000 dilution.

    Lane 1: RAW264.7 cell lysate
    Lane 2: CTLL-2 cell lysate
    Lane 3: J774A.1 cell lysate
    Lane 4: Neuro-2a cell lysate (negative)

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 34 kDa
    Observed band size: 35 kDa

    Exposure time: Lane 1: 25 seconds; Lane 2-4: 1 minute; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723036) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Flow cytometric analysis of Neuro-2a (left, negative) and RAW264.7 (right, positive) cells labeling TREX1.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA723036" style="font-weight: bold;text-decoration: underline;">HA723036</a>, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    ☑ Relative expression (RE)

    Flow cytometric analysis of Neuro-2a (left, negative) and RAW264.7 (right, positive) cells labeling TREX1.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA723036, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

  • TREX1 was immunoprecipitated from 0.2 mg RAW264.7 cell lysate with <a href="/products/HA723036" style="font-weight: bold;text-decoration: underline;">HA723036</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA723036" style="font-weight: bold;text-decoration: underline;">HA723036</a> at 1/1,000 dilution. Mouse anti Rabbit IgG heavy chain (Fc) secondary antibody (<a href="/products/M1003-7" style="font-weight: bold;text-decoration: underline;">M1003-7</a>) at 1/10,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: RAW264.7 cell lysate (input)<br />Lane 2: <a href="/products/HA723036" style="font-weight: bold;text-decoration: underline;">HA723036</a> IP in RAW264.7 cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA723036" style="font-weight: bold;text-decoration: underline;">HA723036</a> in RAW264.7 cell lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 16 seconds; ECL: <a href="/products/K1801" style="font-weight: bold;text-decoration: underline;">K1801</a>

    TREX1 was immunoprecipitated from 0.2 mg RAW264.7 cell lysate with HA723036 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723036 at 1/1,000 dilution. Mouse anti Rabbit IgG heavy chain (Fc) secondary antibody (M1003-7) at 1/10,000 dilution was used for 1 hour at room temperature.

    Lane 1: RAW264.7 cell lysate (input)
    Lane 2: HA723036 IP in RAW264.7 cell lysate
    Lane 3: Rabbit IgG instead of HA723036 in RAW264.7 cell lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 16 seconds; ECL: K1801

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"