FOXG1 Recombinant Rabbit Monoclonal Antibody [PSH09-08] - BSA and Azide free
Catalog# HA751263
FOXG1 Recombinant Rabbit Monoclonal Antibody [PSH09-08] - BSA and Azide free
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WB
-
IP
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Mouse
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unconjugated
Overview
Product Name
FOXG1 Recombinant Rabbit Monoclonal Antibody [PSH09-08] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within mouse FOXG1 aa 1-200.
Species Reactivity
Mouse
Validated Applications
WB, IP
Target Molecular Weight
Predicted band size: 52 kDa
Positive Control
Neuro-2a cell lysate, Mouse brain tissue lysate.
Conjugation
unconjugated
Clone Number
PSH09-08
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:2,000
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IP
-
1-2μg/sample
Target
Function
Forkhead box protein G1 is a protein that in humans is encoded by the FOXG1 gene. This gene belongs to the forkhead family of transcription factors that is characterized by a distinct forkhead domain. The complete function of this gene has not yet been determined; however, it has been shown to play a role in the development of the brain and telencephalon. Mutations of FOXG1 are the cause of FoxG1 syndrome. FoxG1 syndrome is characterized by microcephaly and brain malformations. It affects most aspects of development and can cause seizures. FOXG1 syndrome is classified as an autism spectrum disorder and was previously considered a variant of Rett syndrome.
Background References
1. Bredenkamp N, Seoighe C, Illing N (Feb 2007). "Comparative evolutionary analysis of the FoxG1 transcription factor from diverse vertebrates identifies conserved recognition sites for microRNA regulation". Dev Genes Evol. 217 (3): 227–33. doi:10.1007/s00427-006-0128-x. PMID 17260156. S2CID 20867411.
2. Ariani F, Hayek G, Rondinella D, Artuso R, Mencarelli MA, Spanhol-Rosseto A, Pollazzon M, Buoni S, Spiga O, Ricciardi S, Meloni I, Longo I, Mari F, Broccoli V, Zappella M, Renieri A (2008). "FOXG1 is responsible for the congenital variant of Rett syndrome". Am. J. Hum. Genet. 83 (1): 89–93. doi:10.1016/j.ajhg.2008.05.015. PMC 2443837. PMID 18571142.
Subcellular Location
Nucleus.
UNIPROT
Synonyms
BF-1 antibody
BF-2 antibody
BF1 antibody
BF2 antibody
Brain factor 1 antibody
Brain factor 2 antibody
FHKL antibody
FKH2 antibody
FKHL1 antibody
FKHL2 antibody
ExpandImages
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Western blot analysis of FOXG1 on different lysates with Rabbit anti-FOXG1 antibody (HA751263) at 1/2,000 dilution.
Lane 1: Neuro-2a cell lysate (20 µg/Lane)
Lane 2: Mouse brain tissue lysate (40 µg/Lane)
Predicted band size: 52 kDa
Observed band size: 58 kDa
Exposure time: Lane 1: 10 seconds; Lane 2: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751263) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
FOXG1 was immunoprecipitated from 0.2 mg Neuro-2a cell lysate with HA751263 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751263 at 1/2,000 dilution. Anti-Rabbit IgG for IP, AlpSdAbs® VHH(HRP) (025-100-005) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: Neuro-2a cell lysate (input)
Lane 2: HA751263 IP in Neuro-2a cell lysate
Lane 3: Rabbit IgG instead of HA751263 in Neuro-2a cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 3 seconds; ECL: K1801
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"