PHOS / PDC Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH09-53] (HA751300)
Catalog# HA751300
Rabbit Monoclonal to PHOS / PDC
Tested for
-
WB
Reacts with
-
Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of PHOS / PDC on different lysates with Rabbit anti-PHOS / PDC antibody (HA751300) at 1/5,000 dilution.
Lane 1: mouse eyeball tissue lysate (40 µg/Lane)
Lane 2: mouse liver tissue lysate (negative) (40 µg/Lane)
Lane 3: rat eyeball tissue lysate (40 µg/Lane)
Lane 4: rat liver tissue lysate (negative) (40 µg/Lane)
Predicted band size: 28 kDa
Observed band size: 33 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751300) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of PHOS / PDC on different lysates with Rabbit anti-PHOS / PDC antibody (HA751300) at 1/5,000 dilution.
Lane 1: mouse eyeball tissue lysate (40 µg/Lane)
Lane 2: mouse liver tissue lysate (negative) (40 µg/Lane)
Lane 3: rat eyeball tissue lysate (40 µg/Lane)
Lane 4: rat liver tissue lysate (negative) (40 µg/Lane)
Predicted band size: 28 kDa
Observed band size: 33 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751300) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.