PHOS / PDC Recombinant Rabbit Monoclonal Antibody [PSH09-53]
Catalog# HA723115
PHOS / PDC Recombinant Rabbit Monoclonal Antibody [PSH09-53]
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
PHOS / PDC Recombinant Rabbit Monoclonal Antibody [PSH09-53]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human PHOS/PDC aa 36-69/246
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 28 kDa
Positive Control
Mouse eyeball tissue lysate, rat eyeball tissue lysate.
Conjugation
unconjugated
Clone Number
PSH09-53
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| WB | IHC-P | |
|---|---|---|
| human |
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| mouse |
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| rat |
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Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:5,000
Target
Function
May participate in the regulation of visual phototransduction or in the integration of photoreceptor metabolism. Inhibits the transcriptional activation activity of the cone-rod homeobox CRX.
Background References
1. Jiang Z, Xiong N, Yan R, Li ST, Liu H, Mao Q, Sun Y, Shen S, Ye L, Gao P, Zhang P, Jia W, Zhang H. PDHX acetylation facilitates tumor progression by disrupting PDC assembly and activating lactylation mediated gene expression. Protein Cell. 2024 Sep 23:pwae052. doi: 10.1093/procel/pwae052. Epub ahead of print. PMID: 39311688.
2. Stacpoole PW. Therapeutic Targeting of the Pyruvate Dehydrogenase Complex/Pyruvate Dehydrogenase Kinase (PDC/PDK) Axis in Cancer. J Natl Cancer Inst. 2017 Nov 1;109(11). doi: 10.1093/jnci/djx071. PMID: 29059435.
Subcellular Location
Cytoplasm, cytosol, Nucleus, Cell projection, cilium, photoreceptor outer segment, Photoreceptor inner segment
Synonyms
33 kDa phototransducing protein antibody
MEKA protein antibody
pdc antibody
PHD antibody
PHOS_HUMAN antibody
Phosducin antibody
Protein MEKA antibody
Images
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☑ Relative expression (RE)
Western blot analysis of PHOS / PDC on different lysates with Rabbit anti-PHOS / PDC antibody (HA723115) at 1/5,000 dilution.
Lane 1: mouse eyeball tissue lysate (40 µg/Lane)
Lane 2: mouse liver tissue lysate (negative) (40 µg/Lane)
Lane 3: rat eyeball tissue lysate (40 µg/Lane)
Lane 4: rat liver tissue lysate (negative) (40 µg/Lane)
Predicted band size: 28 kDa
Observed band size: 33 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723115) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"