PNLDC1 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH09-60] (HA751305)
Catalog# HA751305
Rabbit Monoclonal to PNLDC1
Tested for
-
WB
Reacts with
-
Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of PNLDC1 on different lysates with Rabbit anti-PNLDC1 antibody (HA751305) at 1/2,000 dilution.
Lane 1: 293T cell lysate
Lane 2: LNCaP cell lysate
Lane 3: Raji cell lysate
Lane 4: Mouse testis tissue lysate
Lane 5: Rat testis tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 60 kDa
Observed band size: 60 kDa
Exposure time: Lane 1-3: 1 minute 7 seconds; Lane 4-5: 3 minute s; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751305) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: 293T cell lysate
Lane 2: LNCaP cell lysate
Lane 3: Raji cell lysate
Lane 4: Mouse testis tissue lysate
Lane 5: Rat testis tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 60 kDa
Observed band size: 60 kDa
Exposure time: Lane 1-3: 1 minute 7 seconds; Lane 4-5: 3 minute s; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751305) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.