PNLDC1 Recombinant Rabbit Monoclonal Antibody [PSH09-60]
Catalog# HA723122
PNLDC1 Recombinant Rabbit Monoclonal Antibody [PSH09-60]
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
PNLDC1 Recombinant Rabbit Monoclonal Antibody [PSH09-60]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human PNLDC1 aa 101-250.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 60 kDa
Positive Control
293T cell lysate, LNCaP cell lysate, Raji cell lysate, Mouse testis tissue lysate, Rat testis tissue lysate.
Conjugation
unconjugated
Clone Number
PSH09-60
Reactivity Data
| WB | |
|---|---|
| Human |
|
| Mouse |
|
| Rat |
|
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:2,000
Target
Function
3'-exoribonuclease that has a preference for poly(A) tails of mRNAs, thereby efficiently degrading poly(A) tails. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs and is also used to silence certain maternal mRNAs translationally during oocyte maturation and early embryonic development. May act as a regulator of multipotency in embryonic stem cells (By similarity). Is a critical factor for proper spermatogenesis, involved in pre-piRNAs processing to generate mature piRNAs.
Background References
1. Nagirnaja L et al. Variant PNLDC1, Defective piRNA Processing, and Azoospermia. N Engl J Med. 2021 Aug
2. Ding D et al. PNLDC1 is essential for piRNA 3\' end trimming and transposon silencing during spermatogenesis in mice. Nat Commun. 2017 Oct
Subcellular Location
Endoplasmic reticulum membrane.
Synonyms
PNDC1_HUMAN antibody
PNLDC1 antibody
poly(A)-specific ribonuclease (PARN)-like domain containing 1 antibody
Poly(A)-specific ribonuclease PARN-like domain-containing protein 1 antibody
RP1-195P10.1 antibody
Images
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Western blot analysis of PNLDC1 on different lysates with Rabbit anti-PNLDC1 antibody (HA723122) at 1/2,000 dilution.
Lane 1: 293T cell lysate
Lane 2: LNCaP cell lysate
Lane 3: Raji cell lysate
Lane 4: Mouse testis tissue lysate
Lane 5: Rat testis tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 60 kDa
Observed band size: 60 kDa
Exposure time: Lane 1-3: 1 minute 7 seconds; Lane 4-5: 3 minute s; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723122) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"