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PNLDC1 Recombinant Rabbit Monoclonal Antibody [PSH09-60]

Usd: 385

Specification

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Catalog# HA723122

PNLDC1 Recombinant Rabbit Monoclonal Antibody [PSH09-60]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

PNLDC1 Recombinant Rabbit Monoclonal Antibody [PSH09-60]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human PNLDC1 aa 101-250.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 60 kDa

Positive Control

293T cell lysate, LNCaP cell lysate, Raji cell lysate, Mouse testis tissue lysate, Rat testis tissue lysate.

Conjugation

unconjugated

Clone Number

PSH09-60

Reactivity Data

WB
Human
Tested Tested
Mouse
Tested Tested
Rat
Tested Tested

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

Target

Function

3'-exoribonuclease that has a preference for poly(A) tails of mRNAs, thereby efficiently degrading poly(A) tails. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs and is also used to silence certain maternal mRNAs translationally during oocyte maturation and early embryonic development. May act as a regulator of multipotency in embryonic stem cells (By similarity). Is a critical factor for proper spermatogenesis, involved in pre-piRNAs processing to generate mature piRNAs.

Background References

1. Nagirnaja L et al. Variant PNLDC1, Defective piRNA Processing, and Azoospermia. N Engl J Med. 2021 Aug

2. Ding D et al. PNLDC1 is essential for piRNA 3\' end trimming and transposon silencing during spermatogenesis in mice. Nat Commun. 2017 Oct

Subcellular Location

Endoplasmic reticulum membrane.

UNIPROT

SwissProt: Q8NA58 Human

SwissProt: B2RXZ1 Mouse

Entrez Gene: 361478 Rat

Synonyms

PNDC1_HUMAN antibody

PNLDC1 antibody

poly(A)-specific ribonuclease (PARN)-like domain containing 1 antibody

Poly(A)-specific ribonuclease PARN-like domain-containing protein 1 antibody

RP1-195P10.1 antibody

Images

  • Western blot analysis of PNLDC1 on different lysates with Rabbit anti-PNLDC1 antibody (<a href="/products/HA723122" style="font-weight: bold;text-decoration: underline;">HA723122</a>) at 1/2,000 dilution.<br /><br />Lane 1: 293T cell lysate<br />Lane 2: LNCaP cell lysate<br />Lane 3: Raji cell lysate<br />Lane 4: Mouse testis tissue lysate<br />Lane 5: Rat testis tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 60 kDa<br />Observed band size: 60 kDa<br /><br />Exposure time: Lane 1-3: 1 minute 7 seconds; Lane 4-5: 3 minute s; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723122" style="font-weight: bold;text-decoration: underline;">HA723122</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of PNLDC1 on different lysates with Rabbit anti-PNLDC1 antibody (HA723122) at 1/2,000 dilution.

    Lane 1: 293T cell lysate
    Lane 2: LNCaP cell lysate
    Lane 3: Raji cell lysate
    Lane 4: Mouse testis tissue lysate
    Lane 5: Rat testis tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 60 kDa
    Observed band size: 60 kDa

    Exposure time: Lane 1-3: 1 minute 7 seconds; Lane 4-5: 3 minute s; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723122) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"