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Bone sialoprotein 2 Recombinant Rabbit Monoclonal Antibody [PSH09-64] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751306

Bone sialoprotein 2 Recombinant Rabbit Monoclonal Antibody [PSH09-64] - BSA and Azide free

Application

  • WB

  • IHC-P

  • IHC-Fr

  • IF-Tissue

Reactivity

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

Bone sialoprotein 2 Recombinant Rabbit Monoclonal Antibody [PSH09-64] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within mouse Bone sialoprotein 2 aa 1-324/324

Species Reactivity

Mouse, Rat

Validated Applications

WB, IHC-P, IHC-Fr, IF-Tissue

Molecular Weight

Predicted band size: 36 kDa

Positive Control

Mouse articular cartilage tissue lysate, mouse embryo tissue lysate, rat embryo tissue lysate, mouse embryo tissue, rat embryo tissue.

Conjugation

unconjugated

Clone Number

PSH09-64

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:1,000

  • IHC-Fr

  • 1:500

  • IF-Tissue

  • 1:200

Target

Function

Bone sialoprotein (BSP) is a component of mineralized tissues such as bone, dentin, cementum and calcified cartilage. BSP is a significant component of the bone extracellular matrix and has been suggested to constitute approximately 8% of all non-collagenous proteins found in bone and cementum. BSP, a SIBLING protein, was originally isolated from bovine cortical bone as a 23-kDa glycopeptide with high sialic acid content.The human variant of BSP is called bone sialoprotein 2 also known as cell-binding sialoprotein or integrin-binding sialoprotein and is encoded by the IBSP gene.

Background References

1. Rebecca A. Mosig,John A. Martignetti. Loss of MMP-2 in osteoblasts upregulates osteopontin and bone sialoprotein expression in a circuit regulating bone homeostasis. Disease Models & Mechanisms. 2012;0 (0):0-0. doi:10.1242/dmm.007914

2. Yue Ma,Bing Chen,Boyin Zhang, et al. High expression of integrin-binding sialoprotein (IBSP) is associated with poor prognosis of osteosarcoma. Aging. 2023;0 (0):0-0. doi:10.18632/aging.205235

Subcellular Location

Secreted

UNIPROT

SwissProt: Q61711 Mouse

SwissProt: P13839 Rat

Synonyms

BNSP antibody

Bone sialoprotein 2 antibody

Bone sialoprotein II antibody

BSP antibody

BSP II antibody

BSPII antibody

Cell binding sialoprotein antibody

Cell-binding sialoprotein antibody

IBSP antibody

Integrin binding sialoprotein antibody

Expand

Images

  • Western blot analysis of Bone sialoprotein 2 on different lysates with Rabbit anti-Bone sialoprotein 2 antibody (<a href="/products/HA751306" style="font-weight: bold;text-decoration: underline;">HA751306</a>) at 1/2,000 dilution.<br /><br />Lane 1: mouse articular cartilage tissue lysate <br />Lane 2: mouse embryo tissue lysate<br />Lane 3: rat embryo tissue lysate <br /><br />Lysates/proteins at 40 µg/Lane.<br /><br />Predicted band size: 70 kDa<br />Observed band size: 70 kDa<br /><br />Exposure time: 3 minute ; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751306" style="font-weight: bold;text-decoration: underline;">HA751306</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Bone sialoprotein 2 on different lysates with Rabbit anti-Bone sialoprotein 2 antibody (HA751306) at 1/2,000 dilution.

    Lane 1: mouse articular cartilage tissue lysate
    Lane 2: mouse embryo tissue lysate
    Lane 3: rat embryo tissue lysate

    Lysates/proteins at 40 µg/Lane.

    Predicted band size: 70 kDa
    Observed band size: 70 kDa

    Exposure time: 3 minute ; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751306) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunofluorescence analysis of frozen mouse embryonic tissue with Rabbit anti-Bone sialoprotein 2 antibody (<a href="/products/HA751306" style="font-weight: bold;text-decoration: underline;">HA751306</a>) at 1/500 dilution.<br /><br /><span style="font-weight: bold;">The section was not undergone antigen retrieval.</span> The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (<a href="/products/HA751306" style="font-weight: bold;text-decoration: underline;">HA751306</a>, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

    Immunofluorescence analysis of frozen mouse embryonic tissue with Rabbit anti-Bone sialoprotein 2 antibody (HA751306) at 1/500 dilution.

    The section was not undergone antigen retrieval. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA751306, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

  • Immunohistochemical analysis of paraffin-embedded mouse embryo tissue with Rabbit anti-Bone sialoprotein 2 antibody (<a href="/products/HA751306" style="font-weight: bold;text-decoration: underline;">HA751306</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751306" style="font-weight: bold;text-decoration: underline;">HA751306</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue with Rabbit anti-Bone sialoprotein 2 antibody (HA751306) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751306) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat embryo tissue with Rabbit anti-Bone sialoprotein 2 antibody (<a href="/products/HA751306" style="font-weight: bold;text-decoration: underline;">HA751306</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751306" style="font-weight: bold;text-decoration: underline;">HA751306</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat embryo tissue with Rabbit anti-Bone sialoprotein 2 antibody (HA751306) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751306) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"