Nanos3 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH09-65] (HA751307)
Catalog# HA751307
Rabbit Monoclonal to Nanos3
Tested for
-
WB
-
IF-Cell
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IHC-P
Reacts with
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Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Cell treatment (CT)
Western blot analysis of Nanos3 on different lysates with Rabbit anti-Nanos3 antibody (HA751307) at 1/2,000 dilution.
Lane 1: 293T transfected with empty control cell lysate
Lane 2: 293T transfected with Nanos3 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 21 kDa
Observed band size: 21 kDa
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751307) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Nanos3 on different lysates with Rabbit anti-Nanos3 antibody (HA751307) at 1/2,000 dilution.
Lane 1: 293T transfected with empty control cell lysate
Lane 2: 293T transfected with Nanos3 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 21 kDa
Observed band size: 21 kDa
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751307) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.