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RecA Recombinant Rabbit Monoclonal Antibody [PSH09-96] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751327

RecA Recombinant Rabbit Monoclonal Antibody [PSH09-96] - BSA and Azide free

Application

  • WB

  • IF-Cell

Reactivity

  • Escherichia coli

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

RecA Recombinant Rabbit Monoclonal Antibody [PSH09-96] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within becterium RecA aa 1-353.

Species Reactivity

Escherichia coli

Validated Applications

WB, IF-Cell

Molecular Weight

Predicted band size: 38 kDa

Positive Control

E.coli lysates, E.coli.

Conjugation

unconjugated

Clone Number

PSH09-96

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IF-Cell

  • 1:100

Target

Function

RecA is a 38 kilodalton protein essential for the repair and maintenance of DNA in bacteria. Structural and functional homologs to RecA have been found in all kingdoms of life. RecA serves as an archetype for this class of homologous DNA repair proteins. The homologous protein is called RAD51 in eukaryotes and RadA in archaea. RecA has multiple activities, all related to DNA repair. In the bacterial SOS response, it has a co-protease function in the autocatalytic cleavage of the LexA repressor and the λ repressor.

Background References

1. Kaushik V et al. Interaction of RecA mediated SOS response with bacterial persistence, biofilm formation, and host response. Int J Biol Macromol. 2022 Sep

2. Jain K et al. RecA-independent recombination: Dependence on the Escherichia coli RarA protein. Mol Microbiol. 2021 Jun

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: P0A7G6 Escherichia coli

Synonyms

DNA strand exchange and recombination protein with protease and nuclease activity antibody

lexB antibody

Protein recA antibody

recH antibody

Recombinase A antibody

Images

  • Western blot analysis of RecA on E.coli lysates with Rabbit anti-RecA antibody (<a href="/products/HA751327" style="font-weight: bold;text-decoration: underline;">HA751327</a>) at 1/2,000 dilution.<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 38 kDa<br />Observed band size: 42 kDa<br /><br />Exposure time: 25 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751327" style="font-weight: bold;text-decoration: underline;">HA751327</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of RecA on E.coli lysates with Rabbit anti-RecA antibody (HA751327) at 1/2,000 dilution.

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 38 kDa
    Observed band size: 42 kDa

    Exposure time: 25 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751327) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of E.coli labeling RecA with Rabbit anti-RecA antibody (<a href="/products/HA751327" style="font-weight: bold;text-decoration: underline;">HA751327</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-RecA antibody (<a href="/products/HA751327" style="font-weight: bold;text-decoration: underline;">HA751327</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Immunocytochemistry analysis of E.coli labeling RecA with Rabbit anti-RecA antibody (HA751327) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-RecA antibody (HA751327) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"