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VPS34 Recombinant Rabbit Monoclonal Antibody [PSH10-64] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751367

VPS34 Recombinant Rabbit Monoclonal Antibody [PSH10-64] - BSA and Azide free

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

VPS34 Recombinant Rabbit Monoclonal Antibody [PSH10-64] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human VPS34 aa 251-887.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Target Molecular Weight

Predicted band size: 102 kDa

Positive Control

HeLa cell lysate, PC-3 cell lysate, C2C12 cell lysate, C6 cell lysate, Mouse heart tissue lysate, Mouse brain tissue lysate, Rat heart tissue lysate.

Conjugation

unconjugated

Clone Number

PSH10-64

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at 2-8℃. Avoid freeze.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

Target

Function

Class III PI 3-kinase is a subgroup of the enzyme family, phosphoinositide 3-kinase that share a common protein domain structure, substrate specificity and method of activation. There is only one known class III PI 3-kinase, Vps34, which is also the only PI 3-kinase expressed in all eukaryotic cells. In humans it is encoded by the PIK3C3 gene. In human cells Vps34 associates with a regulatory subunit, PIK3R4(p150, Vps15). Vps34 was first identified in a Saccharomyces cerevisiae (budding yeast) screen for proteins involved vesicle-mediated vacuolar protein sorting (hence Vps). A number of proteins containing a phosphoinositide binding domain specific for PtdIns(3)P that function in cellular protein trafficking have been identified.

Background References

1. Jia M et al. ULK1-mediated metabolic reprogramming regulates Vps34 lipid kinase activity by its lactylation. Sci Adv. 2023 Jun

2. Liu Y et al. Targeting VPS34 in autophagy: An update on pharmacological small-molecule compounds. Eur J Med Chem. 2023 Aug

Subcellular Location

Midbody, Late endosome, Cytoplasmic vesicle, autophagosome.

UNIPROT

SwissProt: Q8NEB9 Human

SwissProt: Q6PF93 Mouse

SwissProt: O88763 Rat

Synonyms

hVps34 antibody

MGC61518 antibody

Phosphatidylinositol 3 kinase catalytic subunit type 3 antibody

Phosphatidylinositol 3 kinase class 3 antibody

Phosphatidylinositol 3 kinase p100 subunit antibody

Phosphatidylinositol 3-kinase catalytic subunit type 3 antibody

Phosphatidylinositol 3-kinase p100 subunit antibody

Phosphoinositide 3 kinase class 3 antibody

Phosphoinositide-3-kinase class 3 antibody

PI3 kinase type 3 antibody

Expand

Images

  • This data was developed using <a href="/products/HA723229" style="font-weight: bold;text-decoration: underline;">HA723229</a>, the same antibody clone in a different buffer formulation.<br />Western blot analysis of VPS34 on different lysates with Rabbit anti-VPS34 antibody (<a href="/products/HA723229" style="font-weight: bold;text-decoration: underline;">HA723229</a>) at 1/25,000 dilution.<br /><br />Lane 1: HeLa cell lysate (20 µg/Lane)<br />Lane 2: PC-3 cell lysate (20 µg/Lane)<br />Lane 3: C2C12 cell lysate (20 µg/Lane)<br />Lane 4: C6 cell lysate (20 µg/Lane)<br />Lane 5: Mouse heart tissue lysate (40 µg/Lane)<br />Lane 6: Mouse brain tissue lysate (40 µg/Lane)<br />Lane 7: Rat heart tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 102 kDa<br />Observed band size: 98 kDa<br /><br />Exposure time: 15 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723229" style="font-weight: bold;text-decoration: underline;">HA723229</a>) at 1/25,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    This data was developed using HA723229, the same antibody clone in a different buffer formulation.
    Western blot analysis of VPS34 on different lysates with Rabbit anti-VPS34 antibody (HA723229) at 1/25,000 dilution.

    Lane 1: HeLa cell lysate (20 µg/Lane)
    Lane 2: PC-3 cell lysate (20 µg/Lane)
    Lane 3: C2C12 cell lysate (20 µg/Lane)
    Lane 4: C6 cell lysate (20 µg/Lane)
    Lane 5: Mouse heart tissue lysate (40 µg/Lane)
    Lane 6: Mouse brain tissue lysate (40 µg/Lane)
    Lane 7: Rat heart tissue lysate (40 µg/Lane)

    Predicted band size: 102 kDa
    Observed band size: 98 kDa

    Exposure time: 15 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723229) at 1/25,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />This data was developed using <a href="/products/HA723229" style="font-weight: bold;text-decoration: underline;">HA723229</a>, the same antibody clone in a different buffer formulation.<br />Western blot analysis of VPS34 on different lysates with Rabbit anti-VPS34 antibody (<a href="/products/HA723229" style="font-weight: bold;text-decoration: underline;">HA723229</a>) at 1/10,000 dilution.<br /><br />Lane 1: HAP1-parental cell lysate<br />Lane 2: HAP1-VPS34 KD cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 102 kDa<br />Observed band size: 98 kDa<br /><br />Exposure time: 4 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723229" style="font-weight: bold;text-decoration: underline;">HA723229</a>) at 1/10,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    This data was developed using HA723229, the same antibody clone in a different buffer formulation.
    Western blot analysis of VPS34 on different lysates with Rabbit anti-VPS34 antibody (HA723229) at 1/10,000 dilution.

    Lane 1: HAP1-parental cell lysate
    Lane 2: HAP1-VPS34 KD cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 102 kDa
    Observed band size: 98 kDa

    Exposure time: 4 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723229) at 1/10,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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