VPS34 Recombinant Rabbit Monoclonal Antibody [PSH10-64]
Overview
Product Name
VPS34 Recombinant Rabbit Monoclonal Antibody [PSH10-64]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human VPS34 aa 251-887.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Molecular Weight
Predicted band size: 102 kDa
Positive Control
HeLa cell lysate, PC-3 cell lysate, C2C12 cell lysate, C6 cell lysate, Mouse heart tissue lysate, Mouse brain tissue lysate, Rat heart tissue lysate.
Conjugation
unconjugated
Clone Number
PSH10-64
Reactivity Data
| WB | |
|---|---|
| Human |
|
| Mouse |
|
| Rat |
|
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:2,000-1:5,000
Target
Function
Class III PI 3-kinase is a subgroup of the enzyme family, phosphoinositide 3-kinase that share a common protein domain structure, substrate specificity and method of activation. There is only one known class III PI 3-kinase, Vps34, which is also the only PI 3-kinase expressed in all eukaryotic cells. In humans it is encoded by the PIK3C3 gene. In human cells Vps34 associates with a regulatory subunit, PIK3R4(p150, Vps15). Vps34 was first identified in a Saccharomyces cerevisiae (budding yeast) screen for proteins involved vesicle-mediated vacuolar protein sorting (hence Vps). A number of proteins containing a phosphoinositide binding domain specific for PtdIns(3)P that function in cellular protein trafficking have been identified.
Background References
1. Jia M et al. ULK1-mediated metabolic reprogramming regulates Vps34 lipid kinase activity by its lactylation. Sci Adv. 2023 Jun
2. Liu Y et al. Targeting VPS34 in autophagy: An update on pharmacological small-molecule compounds. Eur J Med Chem. 2023 Aug
Subcellular Location
Midbody, Late endosome, Cytoplasmic vesicle, autophagosome.
Synonyms
hVps34 antibody
MGC61518 antibody
Phosphatidylinositol 3 kinase catalytic subunit type 3 antibody
Phosphatidylinositol 3 kinase class 3 antibody
Phosphatidylinositol 3 kinase p100 subunit antibody
Phosphatidylinositol 3-kinase catalytic subunit type 3 antibody
Phosphatidylinositol 3-kinase p100 subunit antibody
Phosphoinositide 3 kinase class 3 antibody
Phosphoinositide-3-kinase class 3 antibody
PI3 kinase type 3 antibody
ExpandImages
-
Western blot analysis of VPS34 on different lysates with Rabbit anti-VPS34 antibody (HA723229) at 1/5,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: PC-3 cell lysate (20 µg/Lane)
Lane 3: C2C12 cell lysate (20 µg/Lane)
Lane 4: C6 cell lysate (20 µg/Lane)
Lane 5: Mouse heart tissue lysate (40 µg/Lane)
Lane 6: Mouse brain tissue lysate (40 µg/Lane)
Lane 7: Rat heart tissue lysate (40 µg/Lane)
Predicted band size: 102 kDa
Observed band size: 98 kDa
Exposure time: 15 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723229) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of VPS34 on different lysates with Rabbit anti-VPS34 antibody (HA723229) at 1/2,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-VPS34 KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 102 kDa
Observed band size: 98 kDa
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723229) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Citation
-
Targeting periodontal inflammatory microenvironment to ameliorate periodontitis: A nitrogen ions implantation technique
Journal: International Immunopharmacology
DOI: 10.1016/j.intimp.2025.116055
IF: 4.7
Application: WB
Reactivity: Mouse
Publish date: 2025 Dec
Products with the same target and pathway
VPS34 Recombinant Rabbit Monoclonal Antibody [PSH10-64] - BSA and Azide free
Application: WB
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
