TMC1 Recombinant Rabbit Monoclonal Antibody [PSH11-12] - BSA and Azide free
Catalog# HA751396
TMC1 Recombinant Rabbit Monoclonal Antibody [PSH11-12] - BSA and Azide free
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WB
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IF-Cell
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Human
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Mouse
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HA723291
含抗保成分
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unconjugated
Overview
Product Name
TMC1 Recombinant Rabbit Monoclonal Antibody [PSH11-12] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human TMC1 aa 1-150.
Species Reactivity
Human, Mouse
Validated Applications
WB, IF-Cell
Molecular Weight
Predicted band size: 88 kDa
Positive Control
Mouse brain tissue lysates.
Conjugation
unconjugated
Clone Number
PSH11-12
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000
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IF-Cell
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1:2,000
Target
Function
Transmembrane channel-like protein 1 is a protein that in humans is encoded by the TMC1 gene. TMC1 contains six transmembrane domains with both the C and N termini on the endoplasmic side of the membrane, as well as a large loop between domains 4 and 5. This topology is similar to that of transient receptor potential channels (TRPs), a family of proteins involved in the perception of senses such as temperature, taste, pressure, and vision. TMC1 has been located in the post-natal mouse cochlea, and knockouts for TMC1 and TMC2 result in both auditory and vestibular deficits (hearing loss and balance issues) indicating TMC1 is a molecular part of auditory transduction.
Background References
1. Cho SH et al. Novel autosomal dominant TMC1 variants linked to hearing loss: insight into protein-lipid interactions. BMC Med Genomics. 2023 Dec
2. Giese APJ et al. Complexes of vertebrate TMC1/2 and CIB2/3 proteins form hair-cell mechanotransduction cation channels. bioRxiv [Preprint]. 2024 Jul
Subcellular Location
Cell membrane.
Synonyms
Transmembrane channel-like protein 1 1 publication
Alternative names
Transmembrane cochlear-expressed protein 1
Gene names
Name
TMC1
Images
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Western blot analysis of TMC1 on mouse brain tissue lysates with Rabbit anti-TMC1 antibody (HA751396) at 1/2,000 dilution.
Lysates/proteins at 40 µg/Lane.
Predicted band size: 88 kDa
Observed band size: 88 kDa
Exposure time: 3 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751396) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Cell treatment (CT)
Western blot analysis of TMC1 on different lysates with Rabbit anti-TMC1 antibody (HA751396) at 1/2,000 dilution.
Lane 1: 293T transfected with empty control cell lysate
Lane 2: 293T transfected with TMC1 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 88 kDa
Observed band size: 88 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751396) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Cell treatment (CT)
Immunocytochemistry analysis of 293T cells labeling TMC1 with Rabbit anti-TMC1 antibody (HA751396) at 1/2,000 dilution.
293T cells, transfected with empty control (top, negative) / Flag-tagged TMC1 (bottom, positive) expression vector, respectively, were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TMC1 antibody (HA751396) at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Flag (HA601167, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"