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SMOC2 Recombinant Rabbit Monoclonal Antibody [PSH11-25] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751404

SMOC2 Recombinant Rabbit Monoclonal Antibody [PSH11-25] - BSA and Azide free

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

SMOC2 Recombinant Rabbit Monoclonal Antibody [PSH11-25] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within mouse SMOC2 aa 1-447.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 50 kDa

Positive Control

NIH/3T3 cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, human liver cancer tissue.

Conjugation

unconjugated

Clone Number

PSH11-25

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:200

Target

Function

SPARC-related modular calcium-binding protein 2 is a protein that in humans is encoded by the SMOC2 gene. This gene has been shown mutated in clinical cases of major dental developmental defects. Brachycephalic dogs show a shortening of the snout along with a widening of the hard palate. This skull form is highly associated with disorders of breathing and of the eyes. Brachycephaly in dogs is correlated to a retrotransposon induced missplicing the SMOC2 gene.

Background References

1. Liu D et al. SMOC2 promotes aggressive behavior of fibroblast-like synoviocytes in rheumatoid arthritis through transcriptional and post-transcriptional regulating MYO1C. Cell Death Dis. 2022 Dec

2. Ren Y et al. SMOC2 plays a role in heart failure via regulating TGF-beta1/Smad3 pathway-mediated autophagy. Open Med (Wars). 2023 Jul

Subcellular Location

Secreted, extracellular space, extracellular matrix, basement membrane.

UNIPROT

SwissProt: Q9H3U7 Human

SwissProt: Q8CD91 Mouse

Entrez Gene: 292401 Rat

Synonyms

bA270C4A.1 antibody

bA37D8.1 (secreted modular calcium binding protein 2) antibody

bA37D8.1 antibody

dJ421D16.1 (novel thyroglobulin type 1 repeat containing protein) antibody

dJ421D16.1 antibody

MST117 antibody

MSTP117 antibody

MSTP140 antibody

Secreted modular calcium binding protein 2 antibody

Secreted modular calcium-binding protein 2 antibody

Expand

Images

  • Western blot analysis of SMOC2 on different lysates with Rabbit anti-SMOC2 antibody (<a href="/products/HA751404" style="font-weight: bold;text-decoration: underline;">HA751404</a>) at 1/2,000 dilution.<br /><br />Lane 1: NIH/3T3 cell lysate<br />Lane 2: Mouse brain tissue lysate<br />Lane 3: Rat brain tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 50 kDa<br />Observed band size: 55 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751404" style="font-weight: bold;text-decoration: underline;">HA751404</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of SMOC2 on different lysates with Rabbit anti-SMOC2 antibody (HA751404) at 1/2,000 dilution.

    Lane 1: NIH/3T3 cell lysate
    Lane 2: Mouse brain tissue lysate
    Lane 3: Rat brain tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 50 kDa
    Observed band size: 55 kDa

    Exposure time: 3 minutes; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751404) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue with Rabbit anti-SMOC2 antibody (<a href="/products/HA751404" style="font-weight: bold;text-decoration: underline;">HA751404</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751404" style="font-weight: bold;text-decoration: underline;">HA751404</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue with Rabbit anti-SMOC2 antibody (HA751404) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751404) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"