PGAM5 Recombinant Rabbit Monoclonal Antibody [PSH11-68] - BSA and Azide free
Catalog# HA751424
PGAM5 Recombinant Rabbit Monoclonal Antibody [PSH11-68] - BSA and Azide free
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WB
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Human
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Mouse
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Rat
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HA723364
含抗保成分
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unconjugated
Overview
Product Name
PGAM5 Recombinant Rabbit Monoclonal Antibody [PSH11-68] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human PGAM5 aa 30-289.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Molecular Weight
Predicted band size: 32 kDa
Positive Control
HeLa cell lysate, A549cell lysate, HepG2 cell lysate, PANC-1 cell lysate, NIH/3T3 cell lysate, MEF cell lysate, C6 cell lysate.
Conjugation
unconjugated
Clone Number
PSH11-68
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:10,000
Target
Function
PGAM family member 5 (PGAM5), also known as mitochondrial Serine/threonine-protein phosphatase PGAM5 is related to phosphoglycerate mutase family. It, in humans, is encoded by the PGAM5 gene on chromosome 12.
Background References
1. Cheng M et al. PGAM5: A crucial role in mitochondrial dynamics and programmed cell death. Eur J Cell Biol. 2021 Jan
2. Liu Y et al. Targeted knockdown of PGAM5 in synovial macrophages efficiently alleviates osteoarthritis. Bone Res. 2024 Mar
Subcellular Location
Mitochondrion outer membrane, Mitochondrion inner membrane.
Synonyms
Bcl-XL-binding protein v68 antibody
BXLBv68 antibody
MGC5352 antibody
mitochondrial antibody
PGAM5 antibody
PGAM5_HUMAN antibody
Phosphoglycerate mutase family member 5 antibody
Serine/threonine protein phosphatase PGAM5 mitochondrial antibody
Serine/threonine-protein phosphatase PGAM5 antibody
Images
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Western blot analysis of PGAM5 on different lysates with Rabbit anti-PGAM5 antibody (HA751424) at 1/10,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: A549cell lysate
Lane 3: HepG2 cell lysate
Lane 4: PANC-1 cell lysate
Lane 5: NIH/3T3 cell lysate
Lane 6: MEF cell lysate
Lane 7: C6 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 32 kDa
Observed band size: 32 kDa
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751424) at 1/10,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"