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Twist1 Recombinant Rabbit Monoclonal Antibody [PSH11-98] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751431

Twist1 Recombinant Rabbit Monoclonal Antibody [PSH11-98] - BSA and Azide free

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

Twist1 Recombinant Rabbit Monoclonal Antibody [PSH11-98] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human Twist1 aa 1-50.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Target Molecular Weight

Predicted band size: 21 kDa

Positive Control

SH-SY5Y cell lysate, A375 cell lysate, PC-3M cell lysate, MCF7 cell lysate, HeLa cell lysate, NIH/3T3 cell lysate, E14.0 mouse embryonic brain tissue lysate, Rat embryo tissue lysate, LLC cell lysate, Mouse embryo tissue lysate.

Conjugation

unconjugated

Clone Number

PSH11-98

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at 2-8℃. Avoid freeze.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

Target

Function

Twist-related protein 1 (TWIST1) also known as class A basic helix–loop–helix protein 38 (bHLHa38) is a basic helix-loop-helix transcription factor that in humans is encoded by the TWIST1 gene. Basic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation. The protein encoded by this gene is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Dermo1 (a.k.a. TWIST2). The strongest expression of this mRNA is in placental tissue; in adults, mesodermally derived tissues express this mRNA preferentially. Twist1 is thought to regulate osteogenic lineage.

Background References

1. Wu Q et al. Twist1 regulates macrophage plasticity to promote renal fibrosis through galectin-3. Cell Mol Life Sci. 2022 Feb

2. Guan T et al. Phosphorylation of USP29 by CDK1 Governs TWIST1 Stability and Oncogenic Functions. Adv Sci (Weinh). 2023 Apr

Subcellular Location

Nucleus.

UNIPROT

SwissProt: Q15672 Human

SwissProt: P26687 Mouse

Entrez Gene: 85489 Rat

Synonyms

ACS3 antibody

B-HLH DNA binding protein antibody

bHLHa38 antibody

BPES2 antibody

BPES3 antibody

Class A basic helix-loop-helix protein 38 antibody

CRS antibody

CRS1 antibody

CSO antibody

H-twist antibody

Expand

Images

  • This data was developed using <a href="/products/HA723411" style="font-weight: bold;text-decoration: underline;">HA723411</a>, the same antibody clone in a different buffer formulation.<br />Western blot analysis of Twist1 on different lysates with Rabbit anti-Twist1 antibody (<a href="/products/HA723411" style="font-weight: bold;text-decoration: underline;">HA723411</a>) at 1/2,000 dilution.<br /><br />Lane 1: SH-SY5Y cell lysate<br />Lane 2: A375 cell lysate<br />Lane 3: PC-3M cell lysate<br />Lane 4: MCF7 cell lysate<br />Lane 5: HeLa cell lysate<br />Lane 6: NIH/3T3 cell lysate<br />Lane 7: E14.0 mouse embryonic brain tissue lysate<br />Lane 8: Rat embryo tissue lysate<br />Lane 9: LLC cell lysate<br />Lane 10: Mouse embryo tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 21 kDa<br />Observed band size: 24 kDa<br /><br />Exposure time: Lane 1-8: 1 minute; Lane 9-10: 20 seconds; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723411" style="font-weight: bold;text-decoration: underline;">HA723411</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    This data was developed using HA723411, the same antibody clone in a different buffer formulation.
    Western blot analysis of Twist1 on different lysates with Rabbit anti-Twist1 antibody (HA723411) at 1/2,000 dilution.

    Lane 1: SH-SY5Y cell lysate
    Lane 2: A375 cell lysate
    Lane 3: PC-3M cell lysate
    Lane 4: MCF7 cell lysate
    Lane 5: HeLa cell lysate
    Lane 6: NIH/3T3 cell lysate
    Lane 7: E14.0 mouse embryonic brain tissue lysate
    Lane 8: Rat embryo tissue lysate
    Lane 9: LLC cell lysate
    Lane 10: Mouse embryo tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 21 kDa
    Observed band size: 24 kDa

    Exposure time: Lane 1-8: 1 minute; Lane 9-10: 20 seconds; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723411) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"