Twist1 Recombinant Rabbit Monoclonal Antibody [PSH11-98]
Overview
Product Name
Twist1 Recombinant Rabbit Monoclonal Antibody [PSH11-98]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human Twist1 aa 1-50.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 21 kDa
Positive Control
SH-SY5Y cell lysate, A375 cell lysate, PC-3M cell lysate, MCF7 cell lysate, HeLa cell lysate, NIH/3T3 cell lysate, E14.0 mouse embryonic brain tissue lysate, Rat embryo tissue lysate, LLC cell lysate, Mouse embryo tissue lysate.
Conjugation
unconjugated
Clone Number
PSH11-98
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| WB | IHC-P | IHC-Fr | FC | IF-Cell | |
|---|---|---|---|---|---|
| human |
|
|
|
|
|
| mouse |
|
|
|
|
|
| rat |
|
|
|
|
|
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:2,000
Target
Function
Twist-related protein 1 (TWIST1) also known as class A basic helix–loop–helix protein 38 (bHLHa38) is a basic helix-loop-helix transcription factor that in humans is encoded by the TWIST1 gene. Basic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation. The protein encoded by this gene is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Dermo1 (a.k.a. TWIST2). The strongest expression of this mRNA is in placental tissue; in adults, mesodermally derived tissues express this mRNA preferentially. Twist1 is thought to regulate osteogenic lineage.
Background References
1. Wu Q et al. Twist1 regulates macrophage plasticity to promote renal fibrosis through galectin-3. Cell Mol Life Sci. 2022 Feb
2. Guan T et al. Phosphorylation of USP29 by CDK1 Governs TWIST1 Stability and Oncogenic Functions. Adv Sci (Weinh). 2023 Apr
Subcellular Location
Nucleus.
Synonyms
ACS3 antibody
B-HLH DNA binding protein antibody
bHLHa38 antibody
BPES2 antibody
BPES3 antibody
Class A basic helix-loop-helix protein 38 antibody
CRS antibody
CRS1 antibody
CSO antibody
H-twist antibody
ExpandImages
-
Western blot analysis of Twist1 on different lysates with Rabbit anti-Twist1 antibody (HA723411) at 1/2,000 dilution.
Lane 1: SH-SY5Y cell lysate
Lane 2: A375 cell lysate
Lane 3: PC-3M cell lysate
Lane 4: MCF7 cell lysate
Lane 5: HeLa cell lysate
Lane 6: NIH/3T3 cell lysate
Lane 7: E14.0 mouse embryonic brain tissue lysate
Lane 8: Rat embryo tissue lysate
Lane 9: LLC cell lysate
Lane 10: Mouse embryo tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 21 kDa
Observed band size: 24 kDa
Exposure time: Lane 1-8: 1 minute; Lane 9-10: 20 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723411) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Citation
-
FHL3 promotes pancreatic cancer invasion and metastasis through preventing the ubiquitination degradation of EMT associated transcription factors
Journal: Aging (Albany Ny)
DOI:
IF: 5.52
Application: WB
Reactivity: Human
Publish date: 2020 Jan
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"