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Stanniocalcin 2 / STC-2 Recombinant Rabbit Monoclonal Antibody [PSH12-01] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751434

Stanniocalcin 2 / STC-2 Recombinant Rabbit Monoclonal Antibody [PSH12-01] - BSA and Azide free

Application

  • WB

  • IP

Reactivity

  • Human

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

Stanniocalcin 2 / STC-2 Recombinant Rabbit Monoclonal Antibody [PSH12-01] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human STC-2 aa 1-302.

Species Reactivity

Human

Validated Applications

WB, IP

Target Molecular Weight

Predicted band size: 33 kDa

Positive Control

MCF7 cell lysate, T-47D cell lysate, HepG2 cell lysate, HeLa treated with 10μM BFA for 24 hours cell lysate.

Conjugation

unconjugated

Clone Number

PSH12-01

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at 2-8℃. Avoid freeze.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

Target

Function

Stanniocalcin-2 is a protein that in humans is encoded by the STC2 gene. This gene encodes a secreted, homodimeric glycoprotein that is expressed in a wide variety of tissues and may have autocrine or paracrine functions. The encoded protein has 10 of its 15 cysteine residues conserved among stanniocalcin family members and is phosphorylated by casein kinase 2 exclusively on its serine residues. Its C-terminus contains a cluster of histidine residues which may interact with metal ions. The protein may play a role in the regulation of renal and intestinal calcium and phosphate transport, cell metabolism, or cellular calcium/phosphate homeostasis. Constitutive overexpression of human stanniocalcin 2 in mice resulted in pre-and postnatal growth restriction, reduced bone and skeletal muscle growth, and organomegaly. Expression of this gene is induced by estrogen and altered in some breast cancers.

Background References

1. Jiang K et al. STC2 activates PRMT5 to induce radioresistance through DNA damage repair and ferroptosis pathways in esophageal squamous cell carcinoma. Redox Biol. 2023 Apr

2. Qie S et al. Stanniocalcin 2 (STC2): a universal tumour biomarker and a potential therapeutical target. J Exp Clin Cancer Res. 2022 May

Subcellular Location

Secreted.

UNIPROT

SwissProt: O76061 Human

Synonyms

Stanniocalcin 2 antibody

Stanniocalcin related protein antibody

Stanniocalcin-2 antibody

Stanniocalcin-related protein antibody

Stanniocalcin2 antibody

STC 2 antibody

STC related protein antibody

STC-2 antibody

STC-related protein antibody

STC2 antibody

Expand

Images

  • This data was developed using <a href="/products/HA723414" style="font-weight: bold;text-decoration: underline;">HA723414</a>, the same antibody clone in a different buffer formulation.<br />Western blot analysis of Stanniocalcin 2 / STC-2 on different lysates with Rabbit anti-Stanniocalcin 2 / STC-2 antibody (<a href="/products/HA723414" style="font-weight: bold;text-decoration: underline;">HA723414</a>) at 1/2,000 dilution.<br /><br />Lane 1: MCF7 cell lysate<br />Lane 2: T-47D cell lysate<br />Lane 3: HepG2 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 33 kDa<br />Observed band size: 36/40 kDa<br /><br />Exposure time: Lane 1-2: 25 seconds; Lane 3: 1 minute 50 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723414" style="font-weight: bold;text-decoration: underline;">HA723414</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    This data was developed using HA723414, the same antibody clone in a different buffer formulation.
    Western blot analysis of Stanniocalcin 2 / STC-2 on different lysates with Rabbit anti-Stanniocalcin 2 / STC-2 antibody (HA723414) at 1/2,000 dilution.

    Lane 1: MCF7 cell lysate
    Lane 2: T-47D cell lysate
    Lane 3: HepG2 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 33 kDa
    Observed band size: 36/40 kDa

    Exposure time: Lane 1-2: 25 seconds; Lane 3: 1 minute 50 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723414) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />This data was developed using <a href="/products/HA723414" style="font-weight: bold;text-decoration: underline;">HA723414</a>, the same antibody clone in a different buffer formulation.<br />Western blot analysis of Stanniocalcin 2 / STC-2 on different lysates with Rabbit anti-Stanniocalcin 2 / STC-2 antibody (<a href="/products/HA723414" style="font-weight: bold;text-decoration: underline;">HA723414</a>) at 1/2,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: HeLa treated with 10μM BFA for 24 hours cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 33 kDa<br />Observed band size: 36/40 kDa<br /><br />Exposure time: 6 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723414" style="font-weight: bold;text-decoration: underline;">HA723414</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT)

    This data was developed using HA723414, the same antibody clone in a different buffer formulation.
    Western blot analysis of Stanniocalcin 2 / STC-2 on different lysates with Rabbit anti-Stanniocalcin 2 / STC-2 antibody (HA723414) at 1/2,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: HeLa treated with 10μM BFA for 24 hours cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 33 kDa
    Observed band size: 36/40 kDa

    Exposure time: 6 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723414) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • This data was developed using <a href="/products/HA723414" style="font-weight: bold;text-decoration: underline;">HA723414</a>, the same antibody clone in a different buffer formulation.<br />Stanniocalcin 2 / STC-2 was immunoprecipitated from 0.2 mg T-47D cell lysate with <a href="/products/HA723414" style="font-weight: bold;text-decoration: underline;">HA723414</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA723414" style="font-weight: bold;text-decoration: underline;">HA723414</a> at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: T-47D cell lysate (input)<br />Lane 2: <a href="/products/HA723414" style="font-weight: bold;text-decoration: underline;">HA723414</a> IP in T-47D cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA723414" style="font-weight: bold;text-decoration: underline;">HA723414</a> in T-47D cell lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 2 minutes; ECL: <a href="/products/K1801" style="font-weight: bold;text-decoration: underline;">K1801</a>

    This data was developed using HA723414, the same antibody clone in a different buffer formulation.
    Stanniocalcin 2 / STC-2 was immunoprecipitated from 0.2 mg T-47D cell lysate with HA723414 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723414 at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: T-47D cell lysate (input)
    Lane 2: HA723414 IP in T-47D cell lysate
    Lane 3: Rabbit IgG instead of HA723414 in T-47D cell lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 2 minutes; ECL: K1801

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Products with the same target and pathway

metafield image

Stanniocalcin 2 / STC-2 Recombinant Rabbit Monoclonal Antibody [PSH12-01]

Application: WB,IP

Reactivity: Human

Conjugate: unconjugated