Stanniocalcin 2 / STC-2 Recombinant Rabbit Monoclonal Antibody [PSH12-01]
Catalog# HA723414
Stanniocalcin 2 / STC-2 Recombinant Rabbit Monoclonal Antibody [PSH12-01]
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WB
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IP
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Human
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unconjugated
Overview
Product Name
Stanniocalcin 2 / STC-2 Recombinant Rabbit Monoclonal Antibody [PSH12-01]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human STC-2 aa 1-302.
Species Reactivity
Human
Validated Applications
WB, IP
Target Molecular Weight
Predicted band size: 33 kDa
Positive Control
MCF7 cell lysate, T-47D cell lysate, HepG2 cell lysate, HeLa treated with 10μM BFA for 24 hours cell lysate.
Conjugation
unconjugated
Clone Number
PSH12-01
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| WB | IHC-P | FC | IF-Cell | IP | |
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| human |
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| mouse |
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| rat |
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Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000
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IP
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1-2μg/sample
Target
Function
Stanniocalcin-2 is a protein that in humans is encoded by the STC2 gene. This gene encodes a secreted, homodimeric glycoprotein that is expressed in a wide variety of tissues and may have autocrine or paracrine functions. The encoded protein has 10 of its 15 cysteine residues conserved among stanniocalcin family members and is phosphorylated by casein kinase 2 exclusively on its serine residues. Its C-terminus contains a cluster of histidine residues which may interact with metal ions. The protein may play a role in the regulation of renal and intestinal calcium and phosphate transport, cell metabolism, or cellular calcium/phosphate homeostasis. Constitutive overexpression of human stanniocalcin 2 in mice resulted in pre-and postnatal growth restriction, reduced bone and skeletal muscle growth, and organomegaly. Expression of this gene is induced by estrogen and altered in some breast cancers.
Background References
1. Jiang K et al. STC2 activates PRMT5 to induce radioresistance through DNA damage repair and ferroptosis pathways in esophageal squamous cell carcinoma. Redox Biol. 2023 Apr
2. Qie S et al. Stanniocalcin 2 (STC2): a universal tumour biomarker and a potential therapeutical target. J Exp Clin Cancer Res. 2022 May
Subcellular Location
Secreted.
UNIPROT
Synonyms
Stanniocalcin 2 antibody
Stanniocalcin related protein antibody
Stanniocalcin-2 antibody
Stanniocalcin-related protein antibody
Stanniocalcin2 antibody
STC 2 antibody
STC related protein antibody
STC-2 antibody
STC-related protein antibody
STC2 antibody
ExpandImages
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Western blot analysis of Stanniocalcin 2 / STC-2 on different lysates with Rabbit anti-Stanniocalcin 2 / STC-2 antibody (HA723414) at 1/2,000 dilution.
Lane 1: MCF7 cell lysate
Lane 2: T-47D cell lysate
Lane 3: HepG2 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 33 kDa
Observed band size: 36/40 kDa
Exposure time: Lane 1-2: 25 seconds; Lane 3: 1 minute 50 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723414) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Cell treatment (CT)
Western blot analysis of Stanniocalcin 2 / STC-2 on different lysates with Rabbit anti-Stanniocalcin 2 / STC-2 antibody (HA723414) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 10μM BFA for 24 hours cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 33 kDa
Observed band size: 36/40 kDa
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723414) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Stanniocalcin 2 / STC-2 was immunoprecipitated from 0.2 mg T-47D cell lysate with HA723414 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723414 at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: T-47D cell lysate (input)
Lane 2: HA723414 IP in T-47D cell lysate
Lane 3: Rabbit IgG instead of HA723414 in T-47D cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 2 minutes; ECL: K1801
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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