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SLAM / CD150 Recombinant Rabbit Monoclonal Antibody [PSH12-08] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751437

SLAM / CD150 Recombinant Rabbit Monoclonal Antibody [PSH12-08] - BSA and Azide free

Application

  • WB

  • IP

Reactivity

  • Human

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

SLAM / CD150 Recombinant Rabbit Monoclonal Antibody [PSH12-08] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human SLAMF1 aa 1-237.

Species Reactivity

Human

Validated Applications

WB, IP

Target Molecular Weight

Predicted band size: 37 kDa

Positive Control

Raji cell lysate, HUT 102 cell lysate.

Conjugation

unconjugated

Clone Number

PSH12-08

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IP

  • 1-2μg/sample

Target

Function

Signaling lymphocytic activation molecule 1 is a protein that in humans is encoded by the SLAMF1 gene. Recently SLAMF1 has also been designated CD150 (cluster of differentiation 150). SLAMF1 belongs to the signaling lymphocytic activation molecule family. As other receptors from this family, SLAMF1 is expressed in different types of hematopoietic cells and it plays a role in the regulation of the immune system.

Background References

1. Ke BJ et al. Intercellular interaction between FAP+ fibroblasts and CD150+ inflammatory monocytes mediates fibrostenosis in Crohn\'s disease. J Clin Invest. 2024 Jul

2. Hysenaj L et al. CD150-dependent hematopoietic stem cell sensing of Brucella instructs myeloid commitment. J Exp Med. 2023 Jul

Subcellular Location

Cell membrane; Secreted.

UNIPROT

SwissProt: Q13291 Human

Synonyms

4933415F16 antibody

AA177906 antibody

CD 150 antibody

CD150 antibody

CD150 antigen antibody

CDw150 antibody

Estm51 antibody

Ipo 3 antibody

IPO-3 antibody

Ipo3 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of SLAM / CD150 on different lysates with Rabbit anti-SLAM / CD150 antibody (<a href="/products/HA751437" style="font-weight: bold;text-decoration: underline;">HA751437</a>) at 1/2,000 dilution.<br /><br />Lane 1: Raji cell lysate (10 µg/Lane)<br />Lane 2: 293T cell lysate (negative) (30 µg/Lane)<br />Lane 3: HUT 102 cell lysate (30 µg/Lane)<br />Lane 4: HeLa cell lysate (negative) (30 µg/Lane)<br /><br />Predicted band size: 37 kDa<br />Observed band size: 75 kDa<br /><br />Exposure time: 25 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751437" style="font-weight: bold;text-decoration: underline;">HA751437</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of SLAM / CD150 on different lysates with Rabbit anti-SLAM / CD150 antibody (HA751437) at 1/2,000 dilution.

    Lane 1: Raji cell lysate (10 µg/Lane)
    Lane 2: 293T cell lysate (negative) (30 µg/Lane)
    Lane 3: HUT 102 cell lysate (30 µg/Lane)
    Lane 4: HeLa cell lysate (negative) (30 µg/Lane)

    Predicted band size: 37 kDa
    Observed band size: 75 kDa

    Exposure time: 25 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751437) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • SLAM / CD150 was immunoprecipitated from 0.2 mg Raji cell lysate with <a href="/products/HA751437" style="font-weight: bold;text-decoration: underline;">HA751437</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA751437" style="font-weight: bold;text-decoration: underline;">HA751437</a> at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: Raji cell lysate (input)<br />Lane 2: <a href="/products/HA751437" style="font-weight: bold;text-decoration: underline;">HA751437</a> IP in Raji cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA751437" style="font-weight: bold;text-decoration: underline;">HA751437</a> in Raji cell lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 30 seconds; ECL: <a href="/products/K1801" style="font-weight: bold;text-decoration: underline;">K1801</a>

    SLAM / CD150 was immunoprecipitated from 0.2 mg Raji cell lysate with HA751437 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751437 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: Raji cell lysate (input)
    Lane 2: HA751437 IP in Raji cell lysate
    Lane 3: Rabbit IgG instead of HA751437 in Raji cell lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 30 seconds; ECL: K1801

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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metafield image

SLAM / CD150 Recombinant Rabbit Monoclonal Antibody [PSH12-08]

Application: WB,IP

Reactivity: Human

Conjugate: unconjugated