SLAM / CD150 Recombinant Rabbit Monoclonal Antibody [PSH12-08]
Catalog# HA723424
SLAM / CD150 Recombinant Rabbit Monoclonal Antibody [PSH12-08]
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WB
-
IP
-
Human
-
unconjugated
Overview
Product Name
SLAM / CD150 Recombinant Rabbit Monoclonal Antibody [PSH12-08]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human SLAMF1 aa 1-237.
Species Reactivity
Human
Validated Applications
WB, IP
Target Molecular Weight
Predicted band size: 37 kDa
Positive Control
Raji cell lysate, HUT 102 cell lysate.
Conjugation
unconjugated
Clone Number
PSH12-08
Reactivity Data
| WB | IP | |
|---|---|---|
| Human |
|
|
| Mouse |
|
|
| Rat |
|
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:2,000
-
IP
-
1-2μg/sample
Target
Function
Signaling lymphocytic activation molecule 1 is a protein that in humans is encoded by the SLAMF1 gene. Recently SLAMF1 has also been designated CD150 (cluster of differentiation 150). SLAMF1 belongs to the signaling lymphocytic activation molecule family. As other receptors from this family, SLAMF1 is expressed in different types of hematopoietic cells and it plays a role in the regulation of the immune system.
Background References
1. Ke BJ et al. Intercellular interaction between FAP+ fibroblasts and CD150+ inflammatory monocytes mediates fibrostenosis in Crohn\'s disease. J Clin Invest. 2024 Jul
2. Hysenaj L et al. CD150-dependent hematopoietic stem cell sensing of Brucella instructs myeloid commitment. J Exp Med. 2023 Jul
Subcellular Location
Cell membrane; Secreted.
UNIPROT
Synonyms
4933415F16 antibody
AA177906 antibody
CD 150 antibody
CD150 antibody
CD150 antigen antibody
CDw150 antibody
Estm51 antibody
Ipo 3 antibody
IPO-3 antibody
Ipo3 antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of SLAM / CD150 on different lysates with Rabbit anti-SLAM / CD150 antibody (HA723424) at 1/2,000 dilution.
Lane 1: Raji cell lysate (10 µg/Lane)
Lane 2: 293T cell lysate (negative) (30 µg/Lane)
Lane 3: HUT 102 cell lysate (30 µg/Lane)
Lane 4: HeLa cell lysate (negative) (30 µg/Lane)
Predicted band size: 37 kDa
Observed band size: 75 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723424) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
SLAM / CD150 was immunoprecipitated from 0.2 mg Raji cell lysate with HA723424 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723424 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: Raji cell lysate (input)
Lane 2: HA723424 IP in Raji cell lysate
Lane 3: Rabbit IgG instead of HA723424 in Raji cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 30 seconds; ECL: K1801
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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