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ENPP1 / PC1 Recombinant Rabbit Monoclonal Antibody [PSH12-11] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751440

ENPP1 / PC1 Recombinant Rabbit Monoclonal Antibody [PSH12-11] - BSA and Azide free

Application

  • WB

  • IHC-P

Reactivity

  • Human

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

ENPP1 / PC1 Recombinant Rabbit Monoclonal Antibody [PSH12-11] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human ENPP1 aa 576-925.

Species Reactivity

Human

Validated Applications

WB, IHC-P

Target Molecular Weight

Predicted band size: 105 kDa

Positive Control

HepG2 cell lysate, MDA-MB-231 cell lysate, Huh7 cell lysate, human liver tissue.

Conjugation

unconjugated

Clone Number

PSH12-11

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IHC-P

  • 1:1,000

Target

Function

ENPP1 (Ectonucleotide Pyrophosphatase/Phosphodiesterase 1) is a membrane-bound enzyme that hydrolyzes nucleotide triphosphates, such as ATP, into nucleotide monophosphates and pyrophosphate (PPi). It plays a critical role in regulating PPi levels, which are essential for inhibiting bone mineralization and soft tissue calcification. By preventing the growth of hydroxyapatite crystals, ENPP1 helps maintain proper tissue mineralization and prevents ectopic calcification. ENPP1 is involved in multiple physiological processes, including energy metabolism, purinergic signaling, and the regulation of nucleotide sugar availability in the endoplasmic reticulum and Golgi. It also modulates insulin sensitivity and function, contributing to metabolic regulation. Additionally, ENPP1 has been implicated in melanogenesis and the hydrolysis of 2',3'-cGAMP, a secondary messenger involved in immune signaling, although its role in this process remains unclear. Dysregulation or deficiency of ENPP1 is associated with various diseases, including diabetes, obesity, arterial calcification, and autoimmune disorders, making it a significant target for medical research and therapeutic development.

Background References

1. Johnson KA, et al. Osteoblast tissue-nonspecific alkaline phosphatase antagonizes and regulates PC-1. Am J Physiol Regul Integr Comp Physiol. 2000 Oct.

2. Li L, et al. Hydrolysis of 2\'3\'-cGAMP by ENPP1 and design of nonhydrolyzable analogs. Nat Chem Biol. 2014 Dec.

Subcellular Location

Cell membrane, Basolateral cell membrane, Secreted.

UNIPROT

SwissProt: P22413 Human

Synonyms

Alkaline phosphodiesterase 1 antibody

ARHR2 antibody

COLED antibody

E-NPP 1 antibody

Ectonucleotide pyrophosphatase/phosphodiesterase 1 antibody

Ectonucleotide pyrophosphatase/phosphodiesterase family member 1 antibody

ENPP1 antibody

ENPP1_HUMAN antibody

Ly 41 antigen antibody

M6S1 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of ENPP1 / PC1 on different lysates with Rabbit anti-ENPP1 / PC1 antibody (<a href="/products/HA751440" style="font-weight: bold;text-decoration: underline;">HA751440</a>) at 1/1,000 dilution.<br /><br />Lane 1: HepG2 cell lysate<br />Lane 2: MDA-MB-231 cell lysate<br />Lane 3: Huh7 cell lysate<br />Lane 4: THP-1 cell lysate (negative)<br /><br />Lysates/proteins at 20 ug/Lane.<br /><br />Predicted band size: 105 kDa<br />Observed band size: 140 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751440" style="font-weight: bold;text-decoration: underline;">HA751440</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of ENPP1 / PC1 on different lysates with Rabbit anti-ENPP1 / PC1 antibody (HA751440) at 1/1,000 dilution.

    Lane 1: HepG2 cell lysate
    Lane 2: MDA-MB-231 cell lysate
    Lane 3: Huh7 cell lysate
    Lane 4: THP-1 cell lysate (negative)

    Lysates/proteins at 20 ug/Lane.

    Predicted band size: 105 kDa
    Observed band size: 140 kDa

    Exposure time: 10 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751440) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-ENPP1 / PC1 antibody (<a href="/products/HA751440" style="font-weight: bold;text-decoration: underline;">HA751440</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751440" style="font-weight: bold;text-decoration: underline;">HA751440</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-ENPP1 / PC1 antibody (HA751440) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751440) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"