TMEM119 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH12-98] (HA751453)
Catalog# HA751453
Rabbit Monoclonal to TMEM119
Tested for
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WB
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IF-Cell
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FC
-
IP
Reacts with
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Human
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Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of TMEM119 on different lysates with Rabbit anti-TMEM119 antibody (HA751453) at 1/2,000 dilution.
Lane 1: SH-SY5Y cell lysate (20 µg/Lane)
Lane 2: Mouse brain tissue lysate (40 µg/Lane)
Lane 3: Mouse cerebellum tissue lysate (40 µg/Lane)
Lane 4: Rat brain tissue lysate (40 µg/Lane)
Predicted band size: 29 kDa
Observed band size: 35/55 kDa
Exposure time: Lane 1: 3 minutes; Lane 2-4: 14 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751453) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: SH-SY5Y cell lysate (20 µg/Lane)
Lane 2: Mouse brain tissue lysate (40 µg/Lane)
Lane 3: Mouse cerebellum tissue lysate (40 µg/Lane)
Lane 4: Rat brain tissue lysate (40 µg/Lane)
Predicted band size: 29 kDa
Observed band size: 35/55 kDa
Exposure time: Lane 1: 3 minutes; Lane 2-4: 14 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751453) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.