TMEM119 Recombinant Rabbit Monoclonal Antibody [PSH12-98]
Catalog# HA723503
TMEM119 Recombinant Rabbit Monoclonal Antibody [PSH12-98]
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WB
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IF-Cell
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FC
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IP
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
TMEM119 Recombinant Rabbit Monoclonal Antibody [PSH12-98]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within mouse TMEM119 aa 113-280.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, FC, IP
Target Molecular Weight
Predicted band size: 29 kDa
Positive Control
SH-SY5Y cell lysate, Mouse brain tissue lysate, Mouse cerebellum tissue lysate, Rat brain tissue lysate, SH-SY5Y.
Conjugation
unconjugated
Clone Number
PSH12-98
Reactivity Data
| WB | IF-Cell | FC | IP | |
|---|---|---|---|---|
| Human |
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| Mouse |
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| Rat |
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Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000
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IF-Cell
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1:100
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FC
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1:1,000
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IP
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1-2μg/sample
Target
Function
Plays an important role in bone formation and normal bone mineralization. Promotes the differentiation of myoblasts into osteoblasts. May induce the commitment and differentiation of myoblasts into osteoblasts through an enhancement of BMP2 production and interaction with the BMP-RUNX2 pathway. Up-regulates the expression of ATF4, a transcription factor which plays a central role in osteoblast differentiation. Essential for normal spermatogenesis and late testicular differentiation (By similarity).
Background References
1. Kenkhuis B et al. Co-expression patterns of microglia markers Iba1, TMEM119 and P2RY12 in Alzheimer\'s disease. Neurobiol Dis. 2022 Jun
2. Sun T et al. TMEM119 facilitates ovarian cancer cell proliferation, invasion, and migration via the PDGFRB/PI3K/AKT signaling pathway. J Transl Med. 2021 Mar
Subcellular Location
Cell membrane, Cytoplasm, Endoplasmic reticulum membrane, Secreted.
Synonyms
OBIF antibody
Osteoblast induction factor antibody
PSEC0199 antibody
Transmembrane protein 119 antibody
UNQ731/PRO1415 antibody
Images
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Western blot analysis of TMEM119 on different lysates with Rabbit anti-TMEM119 antibody (HA723503) at 1/2,000 dilution.
Lane 1: SH-SY5Y cell lysate (20 µg/Lane)
Lane 2: Mouse brain tissue lysate (40 µg/Lane)
Lane 3: Mouse cerebellum tissue lysate (40 µg/Lane)
Lane 4: Rat brain tissue lysate (40 µg/Lane)
Predicted band size: 29 kDa
Observed band size: 35/55 kDa
Exposure time: Lane 1: 3 minutes; Lane 2-4: 14 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723503) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of SH-SY5Y cells labeling TMEM119 with Rabbit anti-TMEM119 antibody (HA723503) at 1/200 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TMEM119 antibody (HA723503) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Flow cytometric analysis of SH-SY5Y cells labeling TMEM119.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA723503, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). -
TMEM119 was immunoprecipitated from 0.2 mg mouse cerebellum tissue lysate with HA723503 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723503 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: Mouse cerebellum tissue lysate (input)
Lane 2: HA723503 IP in mouse cerebellum tissue lysate
Lane 3: Rabbit IgG instead of HA723503 in mouse cerebellum tissue lysate
Blocking/Dilution buffer: primary antibody dilution (K1803)
Exposure time: 3 minutes; ECL: K1802
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"