ULBP3 Recombinant Rabbit Monoclonal Antibody [PSH13-03] - BSA and Azide free
Catalog# HA751458
ULBP3 Recombinant Rabbit Monoclonal Antibody [PSH13-03] - BSA and Azide free
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WB
-
IHC-P
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IP
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Human
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HA723508
含抗保成分
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unconjugated
Overview
Product Name
ULBP3 Recombinant Rabbit Monoclonal Antibody [PSH13-03] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human ULBP3 aa 21-220.
Species Reactivity
Human
Validated Applications
WB, IHC-P, IP
Molecular Weight
Predicted band size: 28 kDa
Positive Control
MDA-MB-231 cell lysate, MCF7 cell lysate, SW480 cell lysate, HeLa cell lysate, human placenta tissue, human stomach tissue.
Conjugation
unconjugated
Clone Number
PSH13-03
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:5,000
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IHC-P
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1:200
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IP
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1-2μg/sample
Target
Function
UL16 binding protein 3 (ULBP3) is a cell surface glycoprotein encoded by ULBP3 gene located on the chromosome 6. ULBP3 is related to MHC class I molecules, but its gene maps outside the MHC locus. The domain structure of ULBP3 differs significantly from those of conventional MHC class I molecules. It does not contain the α3 domain and the transmembrane segment. ULBP3 is thus composed of only the α1α2 domain which is linked to the cell membrane by the GPI anchor. It functions as a stress-induced ligand for NKG2D receptor.
Background References
1. Zhang KJ et al. LncRNA TYMSOS facilitates breast cancer metastasis and immune escape through downregulating ULBP3. iScience. 2023 Aug
2. Wei L et al. Unveiling the role of the KLF4/Lnc18q22.2/ULBP3 axis in the tumorigenesis and immune escape of hepatocellular carcinoma under hypoxic condition. J Cell Mol Med. 2024 May
Subcellular Location
Cell membrane.
UNIPROT
Synonyms
ALCAN- gamma antibody
ALCAN-gamma antibody
N2DL-3 antibody
N2DL3 antibody
N2DL3_HUMAN antibody
NKG2D ligand 3 antibody
NKG2D ligand 3 precursor antibody
NKG2DL3 antibody
OTTHUMP00000017411 antibody
RAET1N antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of ULBP3 on different lysates with Rabbit anti-ULBP3 antibody (HA751458) at 1/5,000 dilution.
Lane 1: MDA-MB-231 cell lysate (20 µg/Lane)
Lane 2: MCF7 cell lysate (low expression) (20 µg/Lane)
Lane 3: SW480 cell lysate (20 µg/Lane)
Lane 4: HeLa cell lysate (20 µg/Lane)
Predicted band size: 28 kDa
Observed band size: 28 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751458) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-ULBP3 antibody (HA751458) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751458) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human stomach tissue with Rabbit anti-ULBP3 antibody (HA751458) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751458) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
ULBP3 was immunoprecipitated from 0.2 mg HeLa cell lysate with HA751458 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751458 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate (input)
Lane 2: HA751458 IP in HeLa cell lysate
Lane 3: Rabbit IgG instead of HA751458 in HeLa cell lysate
Blocking/Dilution buffer: primary antibody dilution (K1803)
Exposure time: 42 seconds; ECL: K1802 -
Western blot analysis of ULBP3 on different lysates with Rabbit anti-ULBP3 antibody (HA751458) at 1/5,000 dilution.
Lane 1: His-tagged Human ULBP3 recombinant protein
Lane 2: His-tagged Human ULBP1 recombinant protein
Lysates/proteins at 20 ng/Lane.
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751458) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"