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Dlx2 Recombinant Rabbit Monoclonal Antibody [PSH13-47] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751466

Dlx2 Recombinant Rabbit Monoclonal Antibody [PSH13-47] - BSA and Azide free

Application

  • IHC-P

Reactivity

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

Dlx2 Recombinant Rabbit Monoclonal Antibody [PSH13-47] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human Dlx2 aa 1-328.

Species Reactivity

Mouse, Rat

Validated Applications

IHC-P

Molecular Weight

Predicted band size: 34 kDa

Positive Control

Mouse brain tissue, mouse embryo tissue, rat brain tissue, rat embryo tissue.

Conjugation

unconjugated

Clone Number

PSH13-47

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-P

  • 1:40,000

Target

Function

Homeobox protein DLX-2 is a protein that in humans is encoded by the DLX2 gene. Many vertebrate homeo box-containing genes have been identified on the basis of their sequence similarity with Drosophila developmental genes. Members of the Dlx gene family contain a homeobox that is related to that of Distal-less (Dll), a gene expressed in the head and limbs of the developing fruit fly. The Distal-less (Dlx) family of genes comprises at least 6 different members, DLX1-DLX6. The DLX proteins are postulated to play a role in forebrain and craniofacial development. This gene is located in a tail-to-tail configuration with another member of the gene family on the long arm of chromosome 2.

Background References

1. Zhang B et al. DLX2 promotes osteosarcoma epithelial-mesenchymal transition and doxorubicin resistance by enhancing HOXC8-CDH2 axis. iScience. 2023 Oct

2. Zeng X et al. DLX2 activates Wnt1 transcription and mediates Wnt/beta-catenin signal to promote osteogenic differentiation of hBMSCs. Gene. 2020 Jun

Subcellular Location

Nucleus.

UNIPROT

SwissProt: P40764 Mouse

SwissProt: Q64204 Rat

Synonyms

DII A antibody

Distal less homeo box 2 antibody

Distal less homeobox 2 antibody

DLX 2 antibody

Dlx2 antibody

DLX2_HUMAN antibody

Dlx5 antibody

Homeobox protein DLX 2 antibody

Homeobox protein DLX-2 antibody

Homeobox protein DLX2 antibody

Expand

Images

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Dlx2 antibody (<a href="/products/HA751466" style="font-weight: bold;text-decoration: underline;">HA751466</a>) at 1/40,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751466" style="font-weight: bold;text-decoration: underline;">HA751466</a>) at 1/40,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Dlx2 antibody (HA751466) at 1/40,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751466) at 1/40,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse embryo tissue with Rabbit anti-Dlx2 antibody (<a href="/products/HA751466" style="font-weight: bold;text-decoration: underline;">HA751466</a>) at 1/40,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751466" style="font-weight: bold;text-decoration: underline;">HA751466</a>) at 1/40,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue with Rabbit anti-Dlx2 antibody (HA751466) at 1/40,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751466) at 1/40,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Dlx2 antibody (<a href="/products/HA751466" style="font-weight: bold;text-decoration: underline;">HA751466</a>) at 1/40,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751466" style="font-weight: bold;text-decoration: underline;">HA751466</a>) at 1/40,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Dlx2 antibody (HA751466) at 1/40,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751466) at 1/40,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat embryo tissue with Rabbit anti-Dlx2 antibody (<a href="/products/HA751466" style="font-weight: bold;text-decoration: underline;">HA751466</a>) at 1/40,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751466" style="font-weight: bold;text-decoration: underline;">HA751466</a>) at 1/40,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat embryo tissue with Rabbit anti-Dlx2 antibody (HA751466) at 1/40,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751466) at 1/40,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"