TRAF1 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH13-88] (HA751490)
Catalog# HA751490
Rabbit Monoclonal to TRAF1
Tested for
-
WB
-
IP
Reacts with
-
Human
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of TRAF1 on different lysates with Rabbit anti-TRAF1 antibody (HA751490) at 1/10,000 dilution.
Lane 1: Raji cell lysate (20 µg/Lane)
Lane 2: HEK-293 cell lysate (negative) (20 µg/Lane)
Lane 3: Ramos cell lysate (20 µg/Lane)
Lane 4: Daudi cell lysate (20 µg/Lane)
Lane 5: Rat spleen tissue lysate (40 µg/Lane)
Predicted band size: 46 kDa
Observed band size: 50 kDa
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751490) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of TRAF1 on different lysates with Rabbit anti-TRAF1 antibody (HA751490) at 1/10,000 dilution.
Lane 1: Raji cell lysate (20 µg/Lane)
Lane 2: HEK-293 cell lysate (negative) (20 µg/Lane)
Lane 3: Ramos cell lysate (20 µg/Lane)
Lane 4: Daudi cell lysate (20 µg/Lane)
Lane 5: Rat spleen tissue lysate (40 µg/Lane)
Predicted band size: 46 kDa
Observed band size: 50 kDa
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751490) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.