TRAF1 Recombinant Rabbit Monoclonal Antibody [PSH13-88] - BSA and Azide free
Catalog# HA751490
TRAF1 Recombinant Rabbit Monoclonal Antibody [PSH13-88] - BSA and Azide free
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WB
-
IP
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Human
-
Rat
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HA723581
含抗保成分
-
unconjugated
Overview
Product Name
TRAF1 Recombinant Rabbit Monoclonal Antibody [PSH13-88] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human TRAF1 aa 1-200.
Species Reactivity
Human, Rat
Validated Applications
WB, IP
Molecular Weight
Predicted band size: 46 kDa
Positive Control
Raji cell lysate, Ramos cell lysate, Daudi cell lysate, Rat spleen tissue lysate.
Conjugation
unconjugated
Clone Number
PSH13-88
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:10,000
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IP
-
1-2μg/sample
Target
Function
TNF receptor-associated factor 1 is a protein that in humans is encoded by the TRAF1 gene. The protein encoded by this gene is a member of the TNF receptor (TNFR) associated factor (TRAF) protein family. TRAF proteins associate with, and mediate the signal transduction from various receptors of the TNFR superfamily. This protein and TRAF2 form a heterodimeric complex, which is required for TNF-alpha-mediated activation of MAPK8/JNK and NF-kappaB. The protein complex formed by this protein and TRAF2 also interacts with IAP, and thus mediates the anti-apoptotic signals from TNF receptors. The expression of this protein can be induced by Epstein-Barr virus (EBV). EBV infection membrane protein 1 (LMP1) is found to interact with this and other TRAF proteins; this interaction is thought to link LMP1-mediated B lymphocyte transformation to the signal transduction from TNFR family receptors. TRAF1 also functions as a negative regulator of inflammation by interfering with the linear ubiquitination of NEMO downstream of TLR signaling. This explains why TRAF1 polymorphisms cause an increased risk for rheumatic diseases.
Background References
1. Jang H et al. TRAF1 from a Structural Perspective. Biomolecules. 2024 Apr
2. Chen Y et al. N(6)-methyladenosine-modified TRAF1 promotes sunitinib resistance by regulating apoptosis and angiogenesis in a METTL14-dependent manner in renal cell carcinoma. Mol Cancer. 2022 May
Subcellular Location
Cytoplasm, cytosol.
Synonyms
4732496E14Rik antibody
EBI6 antibody
EBV induced protein 6 antibody
Epstein-Barr virus-induced protein 6 antibody
MGC:10353 antibody
Tnf receptor associated factor 1 antibody
TNF receptor-associated factor 1 antibody
TRAF 1 antibody
TRAF1 antibody
TRAF1_HUMAN antibody
Images
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☑ Relative expression (RE)
Western blot analysis of TRAF1 on different lysates with Rabbit anti-TRAF1 antibody (HA751490) at 1/10,000 dilution.
Lane 1: Raji cell lysate (20 µg/Lane)
Lane 2: HEK-293 cell lysate (negative) (20 µg/Lane)
Lane 3: Ramos cell lysate (20 µg/Lane)
Lane 4: Daudi cell lysate (20 µg/Lane)
Lane 5: Rat spleen tissue lysate (40 µg/Lane)
Predicted band size: 46 kDa
Observed band size: 50 kDa
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751490) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
TRAF1 was immunoprecipitated from 0.2 mg Raji cell lysate with HA751490 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751490 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: Raji cell lysate (input)
Lane 2: HA751490 IP in Raji cell lysate
Lane 3: Rabbit IgG instead of HA751490 in Raji cell lysate
Blocking/Dilution buffer: primary antibody dilution (K1803)
Exposure time: 10 seconds; ECL: K1801
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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