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DR3 / LARD Recombinant Rabbit Monoclonal Antibody [PSH13-90] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751492

DR3 / LARD Recombinant Rabbit Monoclonal Antibody [PSH13-90] - BSA and Azide free

Application

  • WB

  • IF-Cell

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

DR3 / LARD Recombinant Rabbit Monoclonal Antibody [PSH13-90] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human DR3 / LARD aa 1-199.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell

Molecular Weight

Predicted band size: 45 kDa

Positive Control

Jurkat cell lysate, K-562 cell lysate, THP-1 cell lysate, HDLM-2 cell lysate, U-87 MG cell lysate, HeLa cell lysate, Neuro-2a cell lysate, NIH/3T3 cell lysate, C6 cell lysate, Mouse spleen tissue lysate, Rat spleen tissue lysate, Jurkat.

Conjugation

unconjugated

Clone Number

PSH13-90

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IF-Cell

  • 1:100

Target

Function

Death receptor 3 (DR3), also known as tumor necrosis factor receptor superfamily member 25 (TNFRSF25), is a cell surface receptor of the tumor necrosis factor receptor superfamily which mediates apoptotic signalling and differentiation. Its only known TNFSF ligand is TNF-like protein 1A (TL1A). The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is expressed preferentially by activated and antigen-experienced T lymphocytes. TNFRSF25 is also highly expressed by FoxP3 positive regulatory T lymphocytes. TNFRSF25 is activated by a monogamous ligand, known as TL1A (TNFSF15), which is rapidly upregulated in antigen presenting cells and some endothelial cells following Toll-Like Receptor or Fc receptor activation. This receptor has been shown to signal both through the TRADD adaptor molecule to stimulate NF-kappa B activity or through the FADD adaptor molecule to stimulate caspase activation and regulate cell apoptosis.

Background References

1. Bamias G et al. Targeting TL1A and DR3: the new frontier of anti-cytokine therapy in IBD. Gut. 2025 Mar

2. Liman N et al. The ever-expanding role of cytokine receptor DR3 in T cells. Cytokine. 2024 Apr

Subcellular Location

Cell membrane; Secreted.

UNIPROT

SwissProt: Q93038 Human

Entrez Gene: 85030 Mouse

Entrez Gene: 500592 Rat

Synonyms

Apo 3 antibody

Apo-3 antibody

Apo3 antibody

Apoptosis inducing receptor AIR antibody

Apoptosis inducing receptor antibody

Apoptosis mediating receptor antibody

Apoptosis mediating receptor DR 3 antibody

Apoptosis mediating receptor DR3 antibody

Apoptosis mediating receptor TRAMP antibody

Apoptosis-inducing receptor AIR antibody

Expand

Images

  • Western blot analysis of DR3 / LARD on different lysates with Rabbit anti-DR3 / LARD antibody (<a href="/products/HA751492" style="font-weight: bold;text-decoration: underline;">HA751492</a>) at 1/5,000 dilution.<br /><br />Lane 1: Jurkat cell lysate (20 µg/Lane)<br />Lane 2: K-562 cell lysate (20 µg/Lane)<br />Lane 3: THP-1 cell lysate (20 µg/Lane)<br />Lane 4: HDLM-2 cell lysate (20 µg/Lane)<br />Lane 5: U-87 MG cell lysate (20 µg/Lane)<br />Lane 6: HeLa cell lysate (20 µg/Lane)<br />Lane 7: Neuro-2a cell lysate (20 µg/Lane)<br />Lane 8: NIH/3T3 cell lysate (20 µg/Lane)<br />Lane 9: C6 cell lysate (20 µg/Lane)<br />Lane 10: Mouse spleen tissue lysate (40 µg/Lane)<br />Lane 11: Rat spleen tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 45 kDa<br />Observed band size: 45 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751492" style="font-weight: bold;text-decoration: underline;">HA751492</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of DR3 / LARD on different lysates with Rabbit anti-DR3 / LARD antibody (HA751492) at 1/5,000 dilution.

    Lane 1: Jurkat cell lysate (20 µg/Lane)
    Lane 2: K-562 cell lysate (20 µg/Lane)
    Lane 3: THP-1 cell lysate (20 µg/Lane)
    Lane 4: HDLM-2 cell lysate (20 µg/Lane)
    Lane 5: U-87 MG cell lysate (20 µg/Lane)
    Lane 6: HeLa cell lysate (20 µg/Lane)
    Lane 7: Neuro-2a cell lysate (20 µg/Lane)
    Lane 8: NIH/3T3 cell lysate (20 µg/Lane)
    Lane 9: C6 cell lysate (20 µg/Lane)
    Lane 10: Mouse spleen tissue lysate (40 µg/Lane)
    Lane 11: Rat spleen tissue lysate (40 µg/Lane)

    Predicted band size: 45 kDa
    Observed band size: 45 kDa

    Exposure time: 10 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751492) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of Jurkat cells labeling DR3 / LARD with Rabbit anti-DR3 / LARD antibody (<a href="/products/HA751492" style="font-weight: bold;text-decoration: underline;">HA751492</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-DR3 / LARD antibody (<a href="/products/HA751492" style="font-weight: bold;text-decoration: underline;">HA751492</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/HA601187" style="font-weight: bold;text-decoration: underline;">HA601187</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of Jurkat cells labeling DR3 / LARD with Rabbit anti-DR3 / LARD antibody (HA751492) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-DR3 / LARD antibody (HA751492) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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metafield image

DR3 / LARD Recombinant Rabbit Monoclonal Antibody [PSH13-90]

Application: WB,IF-Cell

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated