DR3 / LARD Recombinant Rabbit Monoclonal Antibody [PSH13-90]
Catalog# HA723583
DR3 / LARD Recombinant Rabbit Monoclonal Antibody [PSH13-90]
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WB
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IF-Cell
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Human
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Mouse
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Rat
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HA751492
不含抗保成分
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unconjugated
Overview
Product Name
DR3 / LARD Recombinant Rabbit Monoclonal Antibody [PSH13-90]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human DR3 / LARD aa 1-199.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell
Molecular Weight
Predicted band size: 45 kDa
Positive Control
Jurkat cell lysate, K-562 cell lysate, THP-1 cell lysate, HDLM-2 cell lysate, U-87 MG cell lysate, HeLa cell lysate, Neuro-2a cell lysate, NIH/3T3 cell lysate, C6 cell lysate, Mouse spleen tissue lysate, Rat spleen tissue lysate, Jurkat.
Conjugation
unconjugated
Clone Number
PSH13-90
Reactivity Data
| WB | IF-Cell | |
|---|---|---|
| Human |
|
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| Mouse |
|
|
| Rat |
|
|
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:5,000
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IF-Cell
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1:100
Target
Function
Death receptor 3 (DR3), also known as tumor necrosis factor receptor superfamily member 25 (TNFRSF25), is a cell surface receptor of the tumor necrosis factor receptor superfamily which mediates apoptotic signalling and differentiation. Its only known TNFSF ligand is TNF-like protein 1A (TL1A). The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is expressed preferentially by activated and antigen-experienced T lymphocytes. TNFRSF25 is also highly expressed by FoxP3 positive regulatory T lymphocytes. TNFRSF25 is activated by a monogamous ligand, known as TL1A (TNFSF15), which is rapidly upregulated in antigen presenting cells and some endothelial cells following Toll-Like Receptor or Fc receptor activation. This receptor has been shown to signal both through the TRADD adaptor molecule to stimulate NF-kappa B activity or through the FADD adaptor molecule to stimulate caspase activation and regulate cell apoptosis.
Background References
1. Bamias G et al. Targeting TL1A and DR3: the new frontier of anti-cytokine therapy in IBD. Gut. 2025 Mar
2. Liman N et al. The ever-expanding role of cytokine receptor DR3 in T cells. Cytokine. 2024 Apr
Subcellular Location
Cell membrane; Secreted.
Synonyms
Apo 3 antibody
Apo-3 antibody
Apo3 antibody
Apoptosis inducing receptor AIR antibody
Apoptosis inducing receptor antibody
Apoptosis mediating receptor antibody
Apoptosis mediating receptor DR 3 antibody
Apoptosis mediating receptor DR3 antibody
Apoptosis mediating receptor TRAMP antibody
Apoptosis-inducing receptor AIR antibody
ExpandImages
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Western blot analysis of DR3 / LARD on different lysates with Rabbit anti-DR3 / LARD antibody (HA723583) at 1/5,000 dilution.
Lane 1: Jurkat cell lysate (20 µg/Lane)
Lane 2: K-562 cell lysate (20 µg/Lane)
Lane 3: THP-1 cell lysate (20 µg/Lane)
Lane 4: HDLM-2 cell lysate (20 µg/Lane)
Lane 5: U-87 MG cell lysate (20 µg/Lane)
Lane 6: HeLa cell lysate (20 µg/Lane)
Lane 7: Neuro-2a cell lysate (20 µg/Lane)
Lane 8: NIH/3T3 cell lysate (20 µg/Lane)
Lane 9: C6 cell lysate (20 µg/Lane)
Lane 10: Mouse spleen tissue lysate (40 µg/Lane)
Lane 11: Rat spleen tissue lysate (40 µg/Lane)
Predicted band size: 45 kDa
Observed band size: 45 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723583) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of Jurkat cells labeling DR3 / LARD with Rabbit anti-DR3 / LARD antibody (HA723583) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-DR3 / LARD antibody (HA723583) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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