Integrin alpha 1 Recombinant Rabbit Monoclonal Antibody [PSH14-05] - BSA and Azide free
Catalog# HA751500
Integrin alpha 1 Recombinant Rabbit Monoclonal Antibody [PSH14-05] - BSA and Azide free
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WB
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Integrin alpha 1 Recombinant Rabbit Monoclonal Antibody [PSH14-05] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human Integrin alpha 1 aa 630-1,141.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 131 kDa
Positive Control
HeLa cell lysate, Huh7 cell lysate, SW480 cell lysate, HT-29 cell lysate, Mouse small intestine tissue lysate, Rat brain tissue lysate, Neuro-2a cell lysate, human kidney tissue, mouse kidney tissue, rat kidney tissue.
Conjugation
unconjugated
Clone Number
PSH14-05
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| WB | IHC-P | FC | IF-Cell | |
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| human |
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| mouse |
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| rat |
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Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
Integrin alpha-1 also CD49a is an integrin alpha subunit encoded in humans by the gene ITGA1. It makes up half of the α1β1 integrin duplex. Though CD49a can bind a number of ligands including collagen IV, collagen I, and others. CD49a has been implicated as a marker of tissue resident memory T cells, where it may be coexpressed with other markers CD103 and CD69. It has been shown to affect the motility of T cells.
Background References
1. Bayer ML et al. Influence of the integrin alpha-1 subunit and its relationship with high-fat diet upon extracellular matrix synthesis in skeletal muscle and tendon. Cell Tissue Res. 2020 Jul
2. Ren Y et al. ITGA1 Promotes Glioma Cell Proliferation and Affects Immune Cell Infiltration in Low-Grade Glioma. Mediators Inflamm. 2024 Oct
Subcellular Location
Membrane.
Synonyms
CD 49a antibody
CD49 antigen-like family member A antibody
CD49a antibody
CD49a antigen antibody
Integrin alpha-1 antibody
ITA1_HUMAN antibody
Itga 1 antibody
ITGA1 antibody
Laminin and collagen receptor antibody
Very late activation protein 1 antibody
ExpandImages
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This data was developed using HA723595, the same antibody clone in a different buffer formulation.
Western blot analysis of Integrin alpha 1 on different lysates with Rabbit anti-Integrin alpha 1 antibody (HA723595) at 1/5,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: Huh7 cell lysate (20 µg/Lane)
Lane 3: SW480 cell lysate (20 µg/Lane)
Lane 4: HT-29 cell lysate (20 µg/Lane)
Lane 5: Mouse small intestine tissue lysate (40 µg/Lane)
Lane 6: Rat brain tissue lysate (40 µg/Lane)
Lane 7: Neuro-2a cell lysate (20 µg/Lane)
Predicted band size: 131 kDa
Observed band size: 250 kDa
Exposure time: Lane 1-5: 8 seconds; Lane 6-7: 1 minute 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723595) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
This data was developed using HA723595, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Integrin alpha 1 antibody (HA723595) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723595) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA723595, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Integrin alpha 1 antibody (HA723595) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723595) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA723595, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Integrin alpha 1 antibody (HA723595) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723595) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
Integrin alpha 1 Rabbit Polyclonal Antibody
Application: WB,FC
Reactivity: Human,Rat
Conjugate: unconjugated
Integrin alpha 1 Recombinant Rabbit Monoclonal Antibody [PSH14-04]
Application: FC
Reactivity: Human
Conjugate: unconjugated
