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Granzyme B Recombinant Rabbit Monoclonal Antibody [PSH14-20] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751513

Granzyme B Recombinant Rabbit Monoclonal Antibody [PSH14-20] - BSA and Azide free

Application

  • WB

Reactivity

  • Mouse

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

Granzyme B Recombinant Rabbit Monoclonal Antibody [PSH14-20] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Mouse Granzyme B aa 21-247.

Species Reactivity

Mouse

Validated Applications

WB

Molecular Weight

Predicted band size: 27 kDa

Positive Control

CTLL-2 cell lysates.

Conjugation

unconjugated

Clone Number

PSH14-20

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:100,000

Target

Function

Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse . It cleaves after Asp. Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-E (GSDME), releasing the pore-forming moiety of GSDME, thereby triggering pyroptosis and target cell death . Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis .

Background References

1. Gleave A et al. Granzyme B in Autoimmune Skin Disease. Biomolecules. 2023 Feb

2. Jung K et al. Granzyme B in epithelial barrier dysfunction and related skin diseases. Am J Physiol Cell Physiol. 2022 Jul

Subcellular Location

Secreted, Cytolytic granule.

UNIPROT

SwissProt: P04187 Mouse

Synonyms

C11 antibody

Cathepsin G like 1 antibody

Cathepsin G-like 1 antibody

CCPI antibody

CGL 1 antibody

CGL1 antibody

CSP B antibody

CSPB antibody

CTLA 1 antibody

CTLA-1 antibody

Expand

Images

  • Western blot analysis of Granzyme B on CTLL-2 cell lysates with Rabbit anti-Granzyme B antibody (<a href="/products/HA751513" style="font-weight: bold;text-decoration: underline;">HA751513</a>) at 1/100,000 dilution.<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 27 kDa<br />Observed band size: 35 kDa<br /><br />Exposure time: 4 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751513" style="font-weight: bold;text-decoration: underline;">HA751513</a>) at 1/100,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Granzyme B on CTLL-2 cell lysates with Rabbit anti-Granzyme B antibody (HA751513) at 1/100,000 dilution.

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 27 kDa
    Observed band size: 35 kDa

    Exposure time: 4 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751513) at 1/100,000 dilution was used in primary antibody dilution (K1803) at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"