Granzyme B Recombinant Rabbit Monoclonal Antibody [PSH14-20]
Catalog# HA723609
Granzyme B Recombinant Rabbit Monoclonal Antibody [PSH14-20]
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WB
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Mouse
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HA751513
不含抗保成分
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unconjugated
Overview
Product Name
Granzyme B Recombinant Rabbit Monoclonal Antibody [PSH14-20]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Mouse Granzyme B aa 21-247.
Species Reactivity
Mouse
Validated Applications
WB
Molecular Weight
Predicted band size: 27 kDa
Positive Control
CTLL-2 cell lysates.
Conjugation
unconjugated
Clone Number
PSH14-20
Reactivity Data
| WB | |
|---|---|
| Mouse |
|
| Human |
|
| Rat |
|
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:100,000
Target
Function
Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse . It cleaves after Asp. Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-E (GSDME), releasing the pore-forming moiety of GSDME, thereby triggering pyroptosis and target cell death . Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis .
Background References
1. Gleave A et al. Granzyme B in Autoimmune Skin Disease. Biomolecules. 2023 Feb
2. Jung K et al. Granzyme B in epithelial barrier dysfunction and related skin diseases. Am J Physiol Cell Physiol. 2022 Jul
Subcellular Location
Secreted, Cytolytic granule.
UNIPROT
Synonyms
C11 antibody
Cathepsin G like 1 antibody
Cathepsin G-like 1 antibody
CCPI antibody
CGL 1 antibody
CGL1 antibody
CSP B antibody
CSPB antibody
CTLA 1 antibody
CTLA-1 antibody
ExpandImages
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Western blot analysis of Granzyme B on CTLL-2 cell lysates with Rabbit anti-Granzyme B antibody (HA723609) at 1/100,000 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 27 kDa
Observed band size: 35 kDa
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723609) at 1/100,000 dilution was used in primary antibody dilution (K1803) at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"