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Phospho-TAK1 (S412) Recombinant Rabbit Monoclonal Antibody [PSH14-64] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751525

Phospho-TAK1 (S412) Recombinant Rabbit Monoclonal Antibody [PSH14-64] - BSA and Azide free

Application

  • WB

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

Phospho-TAK1 (S412) Recombinant Rabbit Monoclonal Antibody [PSH14-64] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Ser412 of mouse TAK1.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, FC

Molecular Weight

Predicted band size: 67 kDa

Positive Control

K-562 cell lysate, C2C12 cell lysate, C6 cell lysate, HeLa cell lysate, HeLa treated with 100nM Calyculin A for 30 minutes cell lysate, NIH/3T3 cell lysate, NIH/3T3 starved overnight then treated with 100nM Calyculin A for 30 minutes cell lysate, PC-12 cell lysate, PC-12 treated with 100nM Calyculin A for 30 minutes cell lysate.

Conjugation

unconjugated

Clone Number

PSH14-64

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • FC

  • 1:2,000

Target

Function

Mitogen-activated protein kinase kinase kinase 7 (MAP3K7), also known as TAK1, is an enzyme that in humans is encoded by the MAP3K7 gene. This kinase has also been shown to regulate downstream cytokine expression such as TNF. Due to its regulation of TNF, TAK1 has become a novel target for the treatment of TNF mediated diseases such as auto immune disease ( Rheumatoid Arthritis, lupus, IBD) but also other cytokine mediated disorders such as chronic pain and cancer. With the advent of novel selective TAK1 inhibitors, groups have explored the therapeutic potential of TAK1 targeted therapies. One group has shown that the selective TAK1 inhibitor, Takinib developed at Duke University attenuated rheumatoid arthritis like pathology in the CIA mouse model of human inflammatory arthritis. Furthermore, pharmacological inhibition of TAK1 has shown to reduce inflammatory cytokines in particular TNF.

Background References

1. Ma M et al. TAK1 is an essential kinase for STING trafficking. Mol Cell. 2023 Nov

2. Su W et al. TAK1 deficiency promotes liver injury and tumorigenesis via ferroptosis and macrophage cGAS-STING signalling. JHEP Rep. 2023 Feb

Subcellular Location

Cytoplasm, Cell membrane.

UNIPROT

SwissProt: O43318 Human

SwissProt: Q62073 Mouse

SwissProt: P0C8E4 Rat

Synonyms

M3K7_HUMAN antibody

MAP3K 7 antibody

Map3k7 antibody

MEKK7 antibody

Mitogen activated protein kinase kinase kinase 7 antibody

Mitogen-activated protein kinase kinase kinase 7 antibody

TAK1 antibody

TGF beta activated kinase 1 antibody

TGF-beta-activated kinase 1 antibody

TGF1a antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of Phospho-TAK1 (S412) on different lysates with Rabbit anti-Phospho-TAK1 (S412) antibody (<a href="/products/HA751525" style="font-weight: bold;text-decoration: underline;">HA751525</a>) at 1/5,000 dilution.<br /><br />Lane 1: K-562 cell lysate<br />Lane 2: C2C12 cell lysate<br />Lane 3: C6 cell lysate<br />Lane 4: HeLa cell lysate<br />Lane 5: HeLa treated with 100nM Calyculin A for 30 minutes cell lysate<br />Lane 6: NIH/3T3 cell lysate<br />Lane 7: NIH/3T3 starved overnight then treated with 100nM Calyculin A for 30 minutes cell lysate<br />Lane 8: PC-12 cell lysate<br />Lane 9: PC-12 treated with 100nM Calyculin A for 30 minutes cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 67 kDa<br />Observed band size: 67 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751525" style="font-weight: bold;text-decoration: underline;">HA751525</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT)

    Western blot analysis of Phospho-TAK1 (S412) on different lysates with Rabbit anti-Phospho-TAK1 (S412) antibody (HA751525) at 1/5,000 dilution.

    Lane 1: K-562 cell lysate
    Lane 2: C2C12 cell lysate
    Lane 3: C6 cell lysate
    Lane 4: HeLa cell lysate
    Lane 5: HeLa treated with 100nM Calyculin A for 30 minutes cell lysate
    Lane 6: NIH/3T3 cell lysate
    Lane 7: NIH/3T3 starved overnight then treated with 100nM Calyculin A for 30 minutes cell lysate
    Lane 8: PC-12 cell lysate
    Lane 9: PC-12 treated with 100nM Calyculin A for 30 minutes cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 67 kDa
    Observed band size: 67 kDa

    Exposure time: 10 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751525) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Flow cytometric analysis of HeLa cells untreated (left) / treated with 100nM Calyculin A for 30 minutes (right) labeling Phospho-TAK1 (S412).<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA751525" style="font-weight: bold;text-decoration: underline;">HA751525</a>, 1/2,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    ☑ Cell treatment (CT)

    Flow cytometric analysis of HeLa cells untreated (left) / treated with 100nM Calyculin A for 30 minutes (right) labeling Phospho-TAK1 (S412).

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA751525, 1/2,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Flow cytometric analysis of NIH/3T3 cells untreated (left) / treated with 100nM Calyculin A for 30 minutes (right) labeling Phospho-TAK1 (S412).<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA751525" style="font-weight: bold;text-decoration: underline;">HA751525</a>, 1/2,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    ☑ Cell treatment (CT)

    Flow cytometric analysis of NIH/3T3 cells untreated (left) / treated with 100nM Calyculin A for 30 minutes (right) labeling Phospho-TAK1 (S412).

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA751525, 1/2,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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