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Phospho-ELK1 (S383) Recombinant Rabbit Monoclonal Antibody [PSH14-65] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751526

Phospho-ELK1 (S383) Recombinant Rabbit Monoclonal Antibody [PSH14-65] - BSA and Azide free

Application

  • WB

  • IHC-P

  • IP

Reactivity

  • Human

  • Mouse

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

Phospho-ELK1 (S383) Recombinant Rabbit Monoclonal Antibody [PSH14-65] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Ser383 of human ELK1.

Species Reactivity

Human, Mouse

Validated Applications

WB, IHC-P, IP

Molecular Weight

Predicted band size: 45 kDa

Positive Control

RAW264.7 cell lysate, RAW264.7 treated with 10μg/mL LPS for 8 hours cell lysate, human lung tissue untreated / treated.

Conjugation

unconjugated

Clone Number

PSH14-65

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IHC-P

  • 1:1,000

  • IP

  • 1-2μg/sample

Target

Function

ETS Like-1 protein Elk-1 is a protein that in humans is encoded by the ELK1. Elk-1 functions as a transcription activator. It is classified as a ternary complex factor (TCF), a subclass of the ETS family, which is characterized by a common protein domain that regulates DNA binding to target sequences. Elk1 plays important roles in various contexts, including long-term memory formation, drug addiction, Alzheimer's disease, Down syndrome, breast cancer, and depression. Given its role as a transcription factor, Elk1 is expressed in the nuclei of non-neuronal cells. The protein is present in the cytoplasm as well as in the nucleus of mature neurons. In post-mitotic neurons, a variant of Elk1, sElk1, is expressed solely in the nucleus because it lacks the NES site present in the full-length protein. Moreover, while Elk1 is broadly expressed, actual levels vary among tissues. The rat brain, for example, is extremely rich in Elk1, but the protein is exclusively expressed in neurons.

Background References

1. Wei S et al. Elk1 enhances inflammatory cell infiltration and exacerbates acute lung injury/acute respiratory distress syndrome by suppressing Fcgr2b transcription. Mol Med. 2024 Apr

2. Wang J et al. ELK1 suppresses SYTL1 expression by recruiting HDAC2 in bladder cancer progression. Hum Cell. 2022 Nov

Subcellular Location

Nucleus.

UNIPROT

SwissProt: P19419 Human

SwissProt: P41969 Mouse

Synonyms

ELK 1 antibody

Elk1 antibody

ELK1 member of ETS oncogene family antibody

ELK1 protein antibody

ELK1, ETS transcription factor antibody

ELK1_HUMAN antibody

ELK2 member of ETS oncogene family antibody

ETS domain containing protein Elk 1 antibody

ETS domain containing protein Elk1 antibody

ETS domain protein Elk1 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of Phospho-ELK1 (S383) on different lysates with Rabbit anti-Phospho-ELK1 (S383) antibody (<a href="/products/HA751526" style="font-weight: bold;text-decoration: underline;">HA751526</a>) at 1/5,000 dilution.<br /><br />Lane 1: RAW264.7 cell lysate<br />Lane 2: RAW264.7 treated with 10μg/mL LPS for 8 hours cell lysate<br />Lane 3: RAW264.7 treated with 10μg/mL LPS for 8 hours cell lysate, then the membrane treated with λpp for 1 hour<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 45 kDa<br />Observed band size: 55 kDa<br /><br />Exposure time: 59 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751526" style="font-weight: bold;text-decoration: underline;">HA751526</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT)

    Western blot analysis of Phospho-ELK1 (S383) on different lysates with Rabbit anti-Phospho-ELK1 (S383) antibody (HA751526) at 1/5,000 dilution.

    Lane 1: RAW264.7 cell lysate
    Lane 2: RAW264.7 treated with 10μg/mL LPS for 8 hours cell lysate
    Lane 3: RAW264.7 treated with 10μg/mL LPS for 8 hours cell lysate, then the membrane treated with λpp for 1 hour

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 45 kDa
    Observed band size: 55 kDa

    Exposure time: 59 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751526) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human lung tissue untreated / treated with λpp with Rabbit anti-Phospho-ELK1 (S383) antibody (<a href="/products/HA751526" style="font-weight: bold;text-decoration: underline;">HA751526</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751526" style="font-weight: bold;text-decoration: underline;">HA751526</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human lung tissue untreated / treated with λpp with Rabbit anti-Phospho-ELK1 (S383) antibody (HA751526) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751526) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Phospho-ELK1 (S383) was immunoprecipitated from 0.2 mg RAW264.7 treated with 0.1μg/mL LPS for 6 hours cell lysate with <a href="/products/HA751526" style="font-weight: bold;text-decoration: underline;">HA751526</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA751526" style="font-weight: bold;text-decoration: underline;">HA751526</a> at 1/25,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: RAW264.7 treated with 0.1μg/mL LPS for 6 hours cell lysate (input)<br />Lane 2: <a href="/products/HA751526" style="font-weight: bold;text-decoration: underline;">HA751526</a> IP in RAW264.7 treated with 0.1μg/mL LPS for 6 hours cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA751526" style="font-weight: bold;text-decoration: underline;">HA751526</a> in RAW264.7 treated with 0.1μg/mL LPS for 6 hours cell lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 1 minute 2 seconds; ECL: <a href="/products/K1802" style="font-weight: bold;text-decoration: underline;">K1802</a>

    ☑ Cell treatment (CT)

    Phospho-ELK1 (S383) was immunoprecipitated from 0.2 mg RAW264.7 treated with 0.1μg/mL LPS for 6 hours cell lysate with HA751526 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751526 at 1/25,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: RAW264.7 treated with 0.1μg/mL LPS for 6 hours cell lysate (input)
    Lane 2: HA751526 IP in RAW264.7 treated with 0.1μg/mL LPS for 6 hours cell lysate
    Lane 3: Rabbit IgG instead of HA751526 in RAW264.7 treated with 0.1μg/mL LPS for 6 hours cell lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 1 minute 2 seconds; ECL: K1802

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"