Skip to content

TDG Recombinant Rabbit Monoclonal Antibody [PSH10-11] - BSA and Azide free

Usd: 649

Specification

Bulk Order Quote | Customization Request

Catalog# HA751531

TDG Recombinant Rabbit Monoclonal Antibody [PSH10-11] - BSA and Azide free

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

TDG Recombinant Rabbit Monoclonal Antibody [PSH10-11] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human TGD aa 251-410.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 46 kDa

Positive Control

HeLa cell lysate, Jurkat cell lysate, K-562 cell lysate, 293T cell lysate, A431 cell lysate, Mouse lung tissue lysate, Mouse brain tissue lysate, Mouse spleen tissue lysate, Rat brain tissue lysate, mouse lung cancer tissue, mouse kidney tissue, rat kidney tissue.

Conjugation

unconjugated

Clone Number

PSH10-11

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000-1:5,000

  • IHC-P

  • 1:10,000

Target

Function

G/T mismatch-specific thymine DNA glycosylase is an enzyme that in humans is encoded by the TDG gene. Several bacterial proteins have strong sequence homology with this protein. The protein encoded by this gene belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. TDG knockout mouse models showed no increase in mispairing frequency suggesting that other enzymes, like the functional homologue MBD4, may provide functional redundancy. This gene may have a pseudogene in the p arm of chromosome 12.

Background References

1. Ye L et al. Glycosylase-based base editors for efficient T-to-G and C-to-G editing in mammalian cells. Nat Biotechnol. 2024 Oct

Subcellular Location

Nucleus.

UNIPROT

SwissProt: Q13569 Human

SwissProt: P56581 Mouse

Entrez Gene: 114521 Rat

Synonyms

C JUN leucine zipper interactive protein antibody

C JUN leucine zipper interactive protein antibody

C-JUN leucine zipper interactive protein JZA-3 antibody

E130317C12Rik antibody

EC 3.2.2.29 antibody

G/T mismatch specific thymine DNA glycosylase antibody

G/T mismatch specific thymine DNA glycosylase antibody

G/T mismatch-specific thymine DNA glycosylase antibody

JZA 3 antibody

Jza1 antibody

Expand

Images

  • Western blot analysis of TDG on different lysates with Rabbit anti-TDG antibody (<a href="/products/HA751531" style="font-weight: bold;text-decoration: underline;">HA751531</a>) at 1/5,000 dilution.<br /><br />Lane 1: HeLa cell lysate (20 µg/Lane)<br />Lane 2: Jurkat cell lysate (20 µg/Lane)<br />Lane 3: K-562 cell lysate (20 µg/Lane)<br />Lane 4: 293T cell lysate (20 µg/Lane)<br />Lane 5: A431 cell lysate (20 µg/Lane)<br />Lane 6: Mouse lung tissue lysate (40 µg/Lane)<br />Lane 7: Mouse brain tissue lysate (40 µg/Lane)<br />Lane 8: Mouse spleen tissue lysate (40 µg/Lane)<br />Lane 9: Rat brain tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 46 kDa<br />Observed band size: 55 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751531" style="font-weight: bold;text-decoration: underline;">HA751531</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of TDG on different lysates with Rabbit anti-TDG antibody (HA751531) at 1/5,000 dilution.

    Lane 1: HeLa cell lysate (20 µg/Lane)
    Lane 2: Jurkat cell lysate (20 µg/Lane)
    Lane 3: K-562 cell lysate (20 µg/Lane)
    Lane 4: 293T cell lysate (20 µg/Lane)
    Lane 5: A431 cell lysate (20 µg/Lane)
    Lane 6: Mouse lung tissue lysate (40 µg/Lane)
    Lane 7: Mouse brain tissue lysate (40 µg/Lane)
    Lane 8: Mouse spleen tissue lysate (40 µg/Lane)
    Lane 9: Rat brain tissue lysate (40 µg/Lane)

    Predicted band size: 46 kDa
    Observed band size: 55 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751531) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockout (KO)</span><br /><br />Western blot analysis of TDG on different lysates with Rabbit anti-TDG antibody (<a href="/products/HA751531" style="font-weight: bold;text-decoration: underline;">HA751531</a>) at 1/2,000 dilution.<br /><br />Lane 1: B16-parental cell lysate<br />Lane 2: B16-TDG KO cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 56 kDa<br />Observed band size: 55 kDa<br /><br />Exposure time: 16 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751531" style="font-weight: bold;text-decoration: underline;">HA751531</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockout (KO)

    Western blot analysis of TDG on different lysates with Rabbit anti-TDG antibody (HA751531) at 1/2,000 dilution.

    Lane 1: B16-parental cell lysate
    Lane 2: B16-TDG KO cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 56 kDa
    Observed band size: 55 kDa

    Exposure time: 16 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751531) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockout (KO)</span><br /><br />Immunohistochemical analysis of paraffin-embedded mouse lung cancer tissue / TDG KO mouse lung cancer tissue with Rabbit anti-TDG antibody (<a href="/products/HA751531" style="font-weight: bold;text-decoration: underline;">HA751531</a>) at 1/10,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751531" style="font-weight: bold;text-decoration: underline;">HA751531</a>) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    ☑ Knockout (KO)

    Immunohistochemical analysis of paraffin-embedded mouse lung cancer tissue / TDG KO mouse lung cancer tissue with Rabbit anti-TDG antibody (HA751531) at 1/10,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751531) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-TDG antibody (<a href="/products/HA751531" style="font-weight: bold;text-decoration: underline;">HA751531</a>) at 1/10,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751531" style="font-weight: bold;text-decoration: underline;">HA751531</a>) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-TDG antibody (HA751531) at 1/10,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751531) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-TDG antibody (<a href="/products/HA751531" style="font-weight: bold;text-decoration: underline;">HA751531</a>) at 1/10,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751531" style="font-weight: bold;text-decoration: underline;">HA751531</a>) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-TDG antibody (HA751531) at 1/10,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751531) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"