IL-27A p28 Recombinant Rabbit Monoclonal Antibody [PSH14-93] - BSA and Azide free
Catalog# HA751537
IL-27A p28 Recombinant Rabbit Monoclonal Antibody [PSH14-93] - BSA and Azide free
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WB
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Mouse
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unconjugated
Overview
Product Name
IL-27A p28 Recombinant Rabbit Monoclonal Antibody [PSH14-93] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within mouse IL-27A p28 aa 1-234.
Species Reactivity
Mouse
Validated Applications
WB
Target Molecular Weight
Predicted band size: 27 kDa
Positive Control
RAW264.7 treated with 100ng/mL LPS for 4 hours add 1μg/mL BFA for 3 hours cell lysate, RAW264.7 treated with 100nM TPA overnight then treated with 100ng/mL LPS for 4 hours add 1μg/mL BFA for 3 hours cell lysate.
Conjugation
unconjugated
Clone Number
PSH14-93
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| WB | IHC-P | FC | IF-Cell | IP | |
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| human |
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| mouse |
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| rat |
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Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
Interleukin 27 (IL-27) is a member of the IL-12 cytokine family. It is a heterodimeric cytokine that is encoded by two distinct genes, Epstein-Barr virus-induced gene 3 (EBI3) and IL-27p28. IL-27 is expressed by antigen presenting cells and interacts with a specific cell-surface receptor complex known as IL-27 receptor (IL-27R). This receptor consists of two proteins, IL-27Rɑ and gp130. IL-27 induces differentiation of the diverse populations of T cells in the immune system and also upregulates IL-10.
Background References
1. Liu JQ et al. Intratumoral delivery of IL-12 and IL-27 mRNA using lipid nanoparticles for cancer immunotherapy. J Control Release. 2022 May
2. Imamichi T et al. Editorial: IL-27 in health and disease. Front Immunol. 2023 Mar
Subcellular Location
Secreted.
UNIPROT
Synonyms
EBI3 antibody
IL 27 antibody
IL 27 p28 subunit antibody
IL 27A antibody
IL 30 antibody
IL-27 subunit alpha antibody
IL-27-A antibody
IL27 antibody
IL27-A antibody
IL27A antibody
ExpandImages
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☑ Cell treatment (CT)
This data was developed using HA723695, the same antibody clone in a different buffer formulation.
Western blot analysis of IL-27A p28 on different lysates with Rabbit anti-IL-27A p28 antibody (HA723695) at 1/5,000 dilution.
Lane 1: RAW264.7 cell lysate
Lane 2: RAW264.7 treated with 100ng/mL LPS for 4 hours add 1μg/mL BFA for 3 hours cell lysate
Lane 3: RAW264.7 cell lysate
Lane 4: RAW264.7 treated with 100nM TPA overnight then treated with 100ng/mL LPS for 4 hours add 1μg/mL BFA for 3 hours cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 27 kDa
Observed band size: 27 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723695) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"