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CMTM6 Recombinant Rabbit Monoclonal Antibody [PSH15-02] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751540

CMTM6 Recombinant Rabbit Monoclonal Antibody [PSH15-02] - BSA and Azide free

Application

  • WB

  • IP

Reactivity

  • Human

  • Green monkey

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

CMTM6 Recombinant Rabbit Monoclonal Antibody [PSH15-02] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human CMTM6 aa 1-183.

Species Reactivity

Human, Green monkey

Validated Applications

WB, IP

Target Molecular Weight

Predicted band size: 20 kDa

Positive Control

HDLM-2 cell lysate, 293T cell lysate, MDA-MB-231 cell lysate, BxPC-3 cell lysate, Raji cell lysate, COS-1 cell lysate.

Conjugation

unconjugated

Clone Number

PSH15-02

Reactivity Data

Tested Tested Tested
Verified (internally validated)
Published Tested
Reported in literature (not internally validated)
Predicted Tested
Predicted reactive (based on sequence homology)
Not recommended Tested
Not recommended (failed internal validation)
WB IHC-P FC IF-Cell IP
human
Tested Tested
Not Recommended
Not Recommended
Not Recommended
Tested Tested
mouse
Not Recommended
Not Recommended
Not Recommended
Not Recommended
 
rat
Not Recommended
Not Recommended
 
 
 
green monkey
Tested Tested
 
 
 
 

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at 2-8℃. Avoid freeze.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

Target

Function

CKLF like MARVEL transmembrane domain-containing 6 (i.e. CMTM6), previously termed chemokine-like factor superfamily 6 (i.e. CKLFSF6), is a transmembrane protein encoded in humans by the CMTM6 gene (also termed the CKLFSF6, PRO2219, ayoube, or gourari gene). This gene is located in band 22.3 on the short (or "p") arm of chromosome 3. CMTM6 protein belongs to the CKLF-like MARVEL transmembrane domain-containing family of proteins. This family consist of 9 member proteins: CKLF and CMTM1 through CMTM8. The CMTM family proteins are involved in autoimmune diseases, cardiovascular diseases, the male reproductive system, haematopoiesis, and cancer development. CMTM6 protein regulates immune responses to normal and abnormal (particularly cancerous) cells.

Background References

1. Xing F et al. CMTM6 overexpression confers trastuzumab resistance in HER2-positive breast cancer. Mol Cancer. 2023 Jan

2. Liu Q et al. CMTM6 promotes hepatocellular carcinoma progression through stabilizing beta-catenin. Cancer Lett. 2024 Feb

Subcellular Location

Cell membrane, Early endosome membrane, Recycling endosome membrane.

UNIPROT

SwissProt: Q9NX76 Human

Synonyms

chemokine like factor super family 6 antibody

chemokine like factor superfamily 6 antibody

chemokine like factor superfamily member 6 antibody

Chemokine-like factor superfamily member 6 antibody

CKLF like MARVEL transmembrane domain containing 6 antibody

CKLF-like MARVEL transmembrane domain-containing protein 6 antibody

CKLF6_HUMAN antibody

CKLFSF6 antibody

Cmtm6 antibody

Da2 17 antibody

Expand

Images

  • This data was developed using <a href="/products/HA723702" style="font-weight: bold;text-decoration: underline;">HA723702</a>, the same antibody clone in a different buffer formulation.<br />Western blot analysis of CMTM6 on different lysates with Rabbit anti-CMTM6 antibody (<a href="/products/HA723702" style="font-weight: bold;text-decoration: underline;">HA723702</a>) at 1/5,000 dilution.<br /><br />Lane 1: HDLM-2 cell lysate<br />Lane 2: 293T cell lysate<br />Lane 3: MDA-MB-231 cell lysate<br />Lane 4: BxPC-3 cell lysate<br />Lane 5: Raji cell lysate<br />Lane 6: COS-1 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 20 kDa<br />Observed band size: 20 kDa<br /><br />Exposure time: 59 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723702" style="font-weight: bold;text-decoration: underline;">HA723702</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    This data was developed using HA723702, the same antibody clone in a different buffer formulation.
    Western blot analysis of CMTM6 on different lysates with Rabbit anti-CMTM6 antibody (HA723702) at 1/5,000 dilution.

    Lane 1: HDLM-2 cell lysate
    Lane 2: 293T cell lysate
    Lane 3: MDA-MB-231 cell lysate
    Lane 4: BxPC-3 cell lysate
    Lane 5: Raji cell lysate
    Lane 6: COS-1 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 20 kDa
    Observed band size: 20 kDa

    Exposure time: 59 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723702) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • This data was developed using <a href="/products/HA723702" style="font-weight: bold;text-decoration: underline;">HA723702</a>, the same antibody clone in a different buffer formulation.<br />CMTM6 was immunoprecipitated from 0.2 mg MDA-MB-231 cell lysate with <a href="/products/HA723702" style="font-weight: bold;text-decoration: underline;">HA723702</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA723702" style="font-weight: bold;text-decoration: underline;">HA723702</a> at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: MDA-MB-231 cell lysate (input)<br />Lane 2: <a href="/products/HA723702" style="font-weight: bold;text-decoration: underline;">HA723702</a> IP in MDA-MB-231 cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA723702" style="font-weight: bold;text-decoration: underline;">HA723702</a> in MDA-MB-231 cell lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 4 seconds; ECL: <a href="/products/K1801" style="font-weight: bold;text-decoration: underline;">K1801</a>

    This data was developed using HA723702, the same antibody clone in a different buffer formulation.
    CMTM6 was immunoprecipitated from 0.2 mg MDA-MB-231 cell lysate with HA723702 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723702 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: MDA-MB-231 cell lysate (input)
    Lane 2: HA723702 IP in MDA-MB-231 cell lysate
    Lane 3: Rabbit IgG instead of HA723702 in MDA-MB-231 cell lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 4 seconds; ECL: K1801

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"