CMTM6 Recombinant Rabbit Monoclonal Antibody [PSH15-02]
Catalog# HA723702
CMTM6 Recombinant Rabbit Monoclonal Antibody [PSH15-02]
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WB
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IP
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Human
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Green monkey
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unconjugated
Overview
Product Name
CMTM6 Recombinant Rabbit Monoclonal Antibody [PSH15-02]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human CMTM6 aa 1-183.
Species Reactivity
Human, Green monkey
Validated Applications
WB, IP
Target Molecular Weight
Predicted band size: 20 kDa
Positive Control
HDLM-2 cell lysate, 293T cell lysate, MDA-MB-231 cell lysate, BxPC-3 cell lysate, Raji cell lysate, COS-1 cell lysate.
Conjugation
unconjugated
Clone Number
PSH15-02
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| WB | IHC-P | FC | IF-Cell | IP | |
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| human |
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| mouse |
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| rat |
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| green monkey |
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Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:5,000
Target
Function
CKLF like MARVEL transmembrane domain-containing 6 (i.e. CMTM6), previously termed chemokine-like factor superfamily 6 (i.e. CKLFSF6), is a transmembrane protein encoded in humans by the CMTM6 gene (also termed the CKLFSF6, PRO2219, ayoube, or gourari gene). This gene is located in band 22.3 on the short (or "p") arm of chromosome 3. CMTM6 protein belongs to the CKLF-like MARVEL transmembrane domain-containing family of proteins. This family consist of 9 member proteins: CKLF and CMTM1 through CMTM8. The CMTM family proteins are involved in autoimmune diseases, cardiovascular diseases, the male reproductive system, haematopoiesis, and cancer development. CMTM6 protein regulates immune responses to normal and abnormal (particularly cancerous) cells.
Background References
1. Xing F et al. CMTM6 overexpression confers trastuzumab resistance in HER2-positive breast cancer. Mol Cancer. 2023 Jan
2. Liu Q et al. CMTM6 promotes hepatocellular carcinoma progression through stabilizing beta-catenin. Cancer Lett. 2024 Feb
Subcellular Location
Cell membrane, Early endosome membrane, Recycling endosome membrane.
UNIPROT
Synonyms
chemokine like factor super family 6 antibody
chemokine like factor superfamily 6 antibody
chemokine like factor superfamily member 6 antibody
Chemokine-like factor superfamily member 6 antibody
CKLF like MARVEL transmembrane domain containing 6 antibody
CKLF-like MARVEL transmembrane domain-containing protein 6 antibody
CKLF6_HUMAN antibody
CKLFSF6 antibody
Cmtm6 antibody
Da2 17 antibody
ExpandImages
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Western blot analysis of CMTM6 on different lysates with Rabbit anti-CMTM6 antibody (HA723702) at 1/5,000 dilution.
Lane 1: HDLM-2 cell lysate
Lane 2: 293T cell lysate
Lane 3: MDA-MB-231 cell lysate
Lane 4: BxPC-3 cell lysate
Lane 5: Raji cell lysate
Lane 6: COS-1 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 20 kDa
Observed band size: 20 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723702) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
CMTM6 was immunoprecipitated from 0.2 mg MDA-MB-231 cell lysate with HA723702 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723702 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: MDA-MB-231 cell lysate (input)
Lane 2: HA723702 IP in MDA-MB-231 cell lysate
Lane 3: Rabbit IgG instead of HA723702 in MDA-MB-231 cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 4 seconds; ECL: K1801
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"