TCF-4 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH15-19] (HA751548)
Catalog# HA751548
Rabbit Monoclonal to TCF-4
Tested for
-
WB
-
IF-Cell
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IHC-P
Reacts with
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Human
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Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of TCF-4 on different lysates with Rabbit anti-TCF-4 antibody (HA751548) at 1/5,000 dilution.
Lane 1: SH-SY5Y cell lysate
Lane 2: RPMI 8226 cell lysate
Lane 3: A549 cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 71 kDa
Observed band size: 60/75 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751548) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of TCF-4 on different lysates with Rabbit anti-TCF-4 antibody (HA751548) at 1/5,000 dilution.
Lane 1: SH-SY5Y cell lysate
Lane 2: RPMI 8226 cell lysate
Lane 3: A549 cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 71 kDa
Observed band size: 60/75 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751548) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.