Skip to content

TFPI2 Recombinant Rabbit Monoclonal Antibody [PSH15-45] - BSA and Azide free

Usd: 649

Specification

Bulk Order Quote | Customization Request

Catalog# HA751560

TFPI2 Recombinant Rabbit Monoclonal Antibody [PSH15-45] - BSA and Azide free

Application

  • WB

  • IHC-P

  • IP

Reactivity

  • Human

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

TFPI2 Recombinant Rabbit Monoclonal Antibody [PSH15-45] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human TFPI2 aa 1-235.

Species Reactivity

Human

Validated Applications

WB, IHC-P, IP

Molecular Weight

Predicted band size: 27 kDa

Positive Control

U-87 MG cell lysate, human placenta tissue.

Conjugation

unconjugated

Clone Number

PSH15-45

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IHC-P

  • 1:1,000

  • IP

  • 1-2μg/sample

Target

Function

Tissue factor pathway inhibitor 2 is a protein that in humans is encoded by the TFPI2 gene. May play a role in the regulation of plasmin-mediated matrix remodeling. Inhibits trypsin, plasmin, factor VIIa/tissue factor and weakly factor Xa. Has no effect on thrombin.

Background References

1. Pang L et al. Kunitz-type protease inhibitor TFPI2 remodels stemness and immunosuppressive tumor microenvironment in glioblastoma. Nat Immunol. 2023 Oct

2. Guo M et al. The TFPI2-PPARgamma axis induces M2 polarization and inhibits fibroblast activation to promote recovery from post-myocardial infarction in diabetic mice. J Inflamm (Lond). 2023 Nov

Subcellular Location

Secreted.

UNIPROT

SwissProt: P48307 Human

Synonyms

Placental protein 5 antibody

PP5 antibody

REF1 antibody

Retinal pigment epithelium cell factor 1 antibody

TFPI 2 antibody

TFPI-2 antibody

TFPI2 antibody

TFPI2 PEN antibody

TFPI2_HUMAN antibody

Tissue factor pathway inhibitor 2 antibody

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of TFPI2 on different lysates with Rabbit anti-TFPI2 antibody (<a href="/products/HA751560" style="font-weight: bold;text-decoration: underline;">HA751560</a>) at 1/5,000 dilution.<br /><br />Lane 1: U-87 MG cell lysate<br />Lane 2: MCF7 cell lysate (low expression)<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 27 kDa<br />Observed band size: 30 kDa<br /><br />Exposure time: 25 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751560" style="font-weight: bold;text-decoration: underline;">HA751560</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of TFPI2 on different lysates with Rabbit anti-TFPI2 antibody (HA751560) at 1/5,000 dilution.

    Lane 1: U-87 MG cell lysate
    Lane 2: MCF7 cell lysate (low expression)

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 27 kDa
    Observed band size: 30 kDa

    Exposure time: 25 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751560) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of TFPI2 on different lysates with Rabbit anti-TFPI2 antibody (<a href="/products/HA751560" style="font-weight: bold;text-decoration: underline;">HA751560</a>) at 1/2,000 dilution.<br /><br />Lane 1: U-87 MG cell lysate<br />Lane 2: U-87 MG cell lysate treated with deglycosylation<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 27 kDa<br />Observed band size: 27/30 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751560" style="font-weight: bold;text-decoration: underline;">HA751560</a>) at 1/2,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of TFPI2 on different lysates with Rabbit anti-TFPI2 antibody (HA751560) at 1/2,000 dilution.

    Lane 1: U-87 MG cell lysate
    Lane 2: U-87 MG cell lysate treated with deglycosylation

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 27 kDa
    Observed band size: 27/30 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751560) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-TFPI2 antibody (<a href="/products/HA751560" style="font-weight: bold;text-decoration: underline;">HA751560</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751560" style="font-weight: bold;text-decoration: underline;">HA751560</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-TFPI2 antibody (HA751560) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751560) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • TFPI2 was immunoprecipitated from 0.2 mg U-87 MG cell lysate with <a href="/products/HA751560" style="font-weight: bold;text-decoration: underline;">HA751560</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA751560" style="font-weight: bold;text-decoration: underline;">HA751560</a> at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: U-87 MG cell lysate (input)<br />Lane 2: <a href="/products/HA751560" style="font-weight: bold;text-decoration: underline;">HA751560</a> IP in U-87 MG cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA751560" style="font-weight: bold;text-decoration: underline;">HA751560</a> in U-87 MG cell lysate<br /><br />Blocking/Dilution buffer: primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>)<br />Exposure time: 3 minutes; ECL: <a href="/products/K1801" style="font-weight: bold;text-decoration: underline;">K1801</a>

    TFPI2 was immunoprecipitated from 0.2 mg U-87 MG cell lysate with HA751560 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751560 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: U-87 MG cell lysate (input)
    Lane 2: HA751560 IP in U-87 MG cell lysate
    Lane 3: Rabbit IgG instead of HA751560 in U-87 MG cell lysate

    Blocking/Dilution buffer: primary antibody dilution (K1803)
    Exposure time: 3 minutes; ECL: K1801

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Products with the same target and pathway

metafield image

TFPI2 Recombinant Rabbit Monoclonal Antibody [PSH15-45]

Application: WB,IHC-P,IP

Reactivity: Human

Conjugate: unconjugated