TFPI2 Recombinant Rabbit Monoclonal Antibody [PSH15-45]
Catalog# HA723751
TFPI2 Recombinant Rabbit Monoclonal Antibody [PSH15-45]
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WB
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IHC-P
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IP
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Human
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unconjugated
Overview
Product Name
TFPI2 Recombinant Rabbit Monoclonal Antibody [PSH15-45]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human TFPI2 aa 1-235.
Species Reactivity
Human
Validated Applications
WB, IHC-P, IP
Target Molecular Weight
Predicted band size: 27 kDa
Positive Control
U-87 MG cell lysate, human placenta tissue.
Conjugation
unconjugated
Clone Number
PSH15-45
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| WB | IHC-P | FC | IF-Cell | IP | |
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| human |
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| mouse |
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| rat |
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Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:5,000
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IHC-P
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1:1,000
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IP
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1-2μg/sample
Target
Function
Tissue factor pathway inhibitor 2 is a protein that in humans is encoded by the TFPI2 gene. May play a role in the regulation of plasmin-mediated matrix remodeling. Inhibits trypsin, plasmin, factor VIIa/tissue factor and weakly factor Xa. Has no effect on thrombin.
Background References
1. Pang L et al. Kunitz-type protease inhibitor TFPI2 remodels stemness and immunosuppressive tumor microenvironment in glioblastoma. Nat Immunol. 2023 Oct
2. Guo M et al. The TFPI2-PPARgamma axis induces M2 polarization and inhibits fibroblast activation to promote recovery from post-myocardial infarction in diabetic mice. J Inflamm (Lond). 2023 Nov
Subcellular Location
Secreted.
UNIPROT
Synonyms
Placental protein 5 antibody
PP5 antibody
REF1 antibody
Retinal pigment epithelium cell factor 1 antibody
TFPI 2 antibody
TFPI-2 antibody
TFPI2 antibody
TFPI2 PEN antibody
TFPI2_HUMAN antibody
Tissue factor pathway inhibitor 2 antibody
Images
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☑ Relative expression (RE)
Western blot analysis of TFPI2 on different lysates with Rabbit anti-TFPI2 antibody (HA723751) at 1/5,000 dilution.
Lane 1: U-87 MG cell lysate
Lane 2: MCF7 cell lysate (low expression)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 27 kDa
Observed band size: 30 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723751) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of TFPI2 on different lysates with Rabbit anti-TFPI2 antibody (HA723751) at 1/2,000 dilution.
Lane 1: U-87 MG cell lysate
Lane 2: U-87 MG cell lysate treated with deglycosylation
Lysates/proteins at 20 µg/Lane.
Predicted band size: 27 kDa
Observed band size: 27/30 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723751) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-TFPI2 antibody (HA723751) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723751) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
TFPI2 was immunoprecipitated from 0.2 mg U-87 MG cell lysate with HA723751 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723751 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: U-87 MG cell lysate (input)
Lane 2: HA723751 IP in U-87 MG cell lysate
Lane 3: Rabbit IgG instead of HA723751 in U-87 MG cell lysate
Blocking/Dilution buffer: primary antibody dilution (K1803)
Exposure time: 3 minutes; ECL: K1801
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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