MAGED2 Recombinant Rabbit Monoclonal Antibody [PSH15-53] - BSA and Azide free
Catalog# HA751566
MAGED2 Recombinant Rabbit Monoclonal Antibody [PSH15-53] - BSA and Azide free
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WB
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Human
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unconjugated
Overview
Product Name
MAGED2 Recombinant Rabbit Monoclonal Antibody [PSH15-53] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human MAGED2 aa 151-200.
Species Reactivity
Human
Validated Applications
WB
Target Molecular Weight
Predicted band size: 65 kDa
Positive Control
HEK-293 cell lysate, A431 cell lysate, A549 cell lysate, U-2 OS cell lysate.
Conjugation
unconjugated
Clone Number
PSH15-53
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
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Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
Melanoma-associated antigen D2 is a protein that in humans is encoded by the MAGED2 gene. This gene is a member of the MAGED gene family. While the MAGEA and MAGEB genes are silent in normal tissues with the exception of testis and placenta, the MAGED genes are expressed ubiquitously. The MAGED genes are clustered on chromosome Xp11. This gene is located in Xp11.2, a hot spot for X-linked mental retardation (XLMR). Multiple alternatively spliced transcript variants have been found for this gene, however, the full length nature of some variants has not been defined.
Background References
1. Nasrah S et al. MAGED2 Depletion Promotes Stress-Induced Autophagy by Impairing the cAMP/PKA Pathway. Int J Mol Sci. 2023 Aug
2. Reusch B et al. MAGED2 controls vasopressin-induced aquaporin-2 expression in collecting duct cells. J Proteomics. 2022 Feb
Subcellular Location
Cytosol, extracellular region, membrane, nucleolus, nucleoplasm, nucleus, platelet alpha granule lumen.
UNIPROT
Synonyms
11B6 antibody
BCG 1 antibody
BCG-1 antibody
Breast cancer-associated gene 1 protein antibody
HCA10 antibody
Hepatocellular carcinoma-associated protein JCL-1 antibody
JCL 1 antibody
MAGD2_HUMAN antibody
MAGE D antibody
MAGE D2 antigen antibody
ExpandImages
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This data was developed using HA723759, the same antibody clone in a different buffer formulation.
Western blot analysis of MAGED2 on different lysates with Rabbit anti-MAGED2 antibody (HA723759) at 1/1,000 dilution.
Lane 1: HEK-293 cell lysate
Lane 2: A431 cell lysate
Lane 3: A549 cell lysate
Lane 4: U-2 OS cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 65 kDa
Observed band size: 72 kDa
Exposure time: 1 minute 18 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723759) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"