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MAGED2 Recombinant Rabbit Monoclonal Antibody [PSH15-53] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751566

MAGED2 Recombinant Rabbit Monoclonal Antibody [PSH15-53] - BSA and Azide free

Application

  • WB

Reactivity

  • Human

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

MAGED2 Recombinant Rabbit Monoclonal Antibody [PSH15-53] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human MAGED2 aa 151-200.

Species Reactivity

Human

Validated Applications

WB

Molecular Weight

Predicted band size: 65 kDa

Positive Control

HEK-293 cell lysate, A431 cell lysate, A549 cell lysate, U-2 OS cell lysate.

Conjugation

unconjugated

Clone Number

PSH15-53

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

Melanoma-associated antigen D2 is a protein that in humans is encoded by the MAGED2 gene. This gene is a member of the MAGED gene family. While the MAGEA and MAGEB genes are silent in normal tissues with the exception of testis and placenta, the MAGED genes are expressed ubiquitously. The MAGED genes are clustered on chromosome Xp11. This gene is located in Xp11.2, a hot spot for X-linked mental retardation (XLMR). Multiple alternatively spliced transcript variants have been found for this gene, however, the full length nature of some variants has not been defined.

Background References

1. Nasrah S et al. MAGED2 Depletion Promotes Stress-Induced Autophagy by Impairing the cAMP/PKA Pathway. Int J Mol Sci. 2023 Aug

2. Reusch B et al. MAGED2 controls vasopressin-induced aquaporin-2 expression in collecting duct cells. J Proteomics. 2022 Feb

Subcellular Location

Cytosol, extracellular region, membrane, nucleolus, nucleoplasm, nucleus, platelet alpha granule lumen.

UNIPROT

SwissProt: Q9UNF1 Human

Synonyms

11B6 antibody

BCG 1 antibody

BCG-1 antibody

Breast cancer-associated gene 1 protein antibody

HCA10 antibody

Hepatocellular carcinoma-associated protein JCL-1 antibody

JCL 1 antibody

MAGD2_HUMAN antibody

MAGE D antibody

MAGE D2 antigen antibody

Expand

Images

  • Western blot analysis of MAGED2 on different lysates with Rabbit anti-MAGED2 antibody (<a href="/products/HA751566" style="font-weight: bold;text-decoration: underline;">HA751566</a>) at 1/1,000 dilution.<br /><br />Lane 1: HEK-293 cell lysate<br />Lane 2: A431 cell lysate<br />Lane 3: A549 cell lysate<br />Lane 4: U-2 OS cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 65 kDa<br />Observed band size: 72 kDa<br /><br />Exposure time: 1 minute 18 seconds; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751566" style="font-weight: bold;text-decoration: underline;">HA751566</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of MAGED2 on different lysates with Rabbit anti-MAGED2 antibody (HA751566) at 1/1,000 dilution.

    Lane 1: HEK-293 cell lysate
    Lane 2: A431 cell lysate
    Lane 3: A549 cell lysate
    Lane 4: U-2 OS cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 65 kDa
    Observed band size: 72 kDa

    Exposure time: 1 minute 18 seconds; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751566) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"