IL-1 alpha Recombinant Rabbit Monoclonal Antibody [PSH15-66] - BSA and Azide free
Catalog# HA751577
IL-1 alpha Recombinant Rabbit Monoclonal Antibody [PSH15-66] - BSA and Azide free
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IF-Cell
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FC
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Mouse
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HA723773
含抗保成分
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unconjugated
Overview
Product Name
IL-1 alpha Recombinant Rabbit Monoclonal Antibody [PSH15-66] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within mouse IL-1 alpha aa 115-270.
Species Reactivity
Mouse
Validated Applications
IF-Cell, FC
Molecular Weight
Predicted band size: 31 kDa
Positive Control
RAW264.7 cells treated with 10μg/mL LPS for 8 hours.
Conjugation
unconjugated
Clone Number
PSH15-66
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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IF-Cell
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1:3,000
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FC
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1:1,000
Target
Function
Interleukin-1 alpha (IL-1 alpha) also known as hematopoietin 1 is a cytokine of the interleukin 1 family that in humans is encoded by the IL1A gene. In general, Interleukin 1 is responsible for the production of inflammation, as well as the promotion of fever and sepsis. IL-1α inhibitors are being developed to interrupt those processes and treat diseases. IL-1α is produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. It binds to the interleukin-1 receptor. It is on the pathway that activates tumor necrosis factor-alpha.
Background References
1. Park MD et al. Hematopoietic aging promotes cancer by fueling IL-1⍺-driven emergency myelopoiesis. Science. 2024 Oct
2. Parlak E et al. Analysis of Chitinase-3-Like Protein 1, IL-1-Alpha, and IL-6 as Novel Inflammatory Biomarkers for COVID-19. J Interferon Cytokine Res. 2022 Oct
Subcellular Location
Nucleus, Cytoplasm, Secreted.
UNIPROT
Synonyms
BAF antibody
FAF antibody
Hematopoietin 1 antibody
Hematopoietin-1 antibody
IL 1 alpha antibody
IL 1A antibody
IL-1 alpha antibody
Il-1a antibody
IL1 ALPHA antibody
IL1 antibody
ExpandImages
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Immunocytochemistry analysis of RAW264.7 cells untreated / treated with 10μg/mL LPS for 8 hours labeling IL-1 alpha with Rabbit anti-IL-1 alpha antibody (HA751577) at 1/3,000 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IL-1 alpha antibody (HA751577) at 1/3,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Flow cytometric analysis of RAW264.7 cells untreated (left) / treated with 10μg/mL LPS for 8 hours (right) labeling IL-1 alpha.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA751577, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"