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UCP1 Recombinant Rabbit Monoclonal Antibody [PSH15-73] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751580

UCP1 Recombinant Rabbit Monoclonal Antibody [PSH15-73] - BSA and Azide free

Application

  • WB

  • IHC-Fr

  • IHC-P

  • IP

Reactivity

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

UCP1 Recombinant Rabbit Monoclonal Antibody [PSH15-73] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Species Reactivity

Mouse, Rat

Validated Applications

WB, IHC-Fr, IHC-P, IP

Molecular Weight

Predicted band size: 33 kDa

Positive Control

Mouse brown adipose tissue lysate, Rat brown adipose tissue lysate, mouse brown adipose tissue, rat brown adipose tissue.

Conjugation

unconjugated

Clone Number

PSH15-73

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-Fr

  • 1:500

  • IHC-P

  • 1:1,000

  • IP

  • 1-2μg/sample

Target

Function

Thermogenin (called uncoupling protein by its discoverers and now known as uncoupling protein 1, or UCP1) is a mitochondrial carrier protein found in brown adipose tissue (BAT). It is used to generate heat by non-shivering thermogenesis, and makes a quantitatively important contribution to countering heat loss in babies which would otherwise occur due to their high surface area-volume ratio. Recent findings indicate that the UCP1 protein plays a crucial role in thermogenesis by catalyzing the dissipative production of heat through protons derived from NADH and FADH2. These electron carriers are produced in the TCA cycle from the oxidation of acetyl-CoA, which comes from the breakdown of free fatty acids. Intriguingly, the acetyl-CoA products undergo a recycling process that facilitates their re-utilization, thereby sustaining the cycle known as the HEAT cycle.

Background References

1. Rahbani JF et al. Parallel control of cold-triggered adipocyte thermogenesis by UCP1 and CKB. Cell Metab. 2024 Mar

2. Johnson JM et al. Mitochondrial phosphatidylethanolamine modulates UCP1 to promote brown adipose thermogenesis. Sci Adv. 2023 Feb

Subcellular Location

Mitochondrion inner membrane.

UNIPROT

SwissProt: P12242 Mouse

SwissProt: P04633 Rat

Synonyms

mitochondrial brown fat uncoupling protein antibody

Mitochondrial brown fat uncoupling protein 1 antibody

SLC25A7 antibody

Solute carrier family 25 member 7 antibody

Thermogenin antibody

UCP 1 antibody

UCP antibody

UCP1 antibody

UCP1_HUMAN antibody

uncoupling protein 1 (mitochondrial, proton carrier) antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of UCP1 on different lysates with Rabbit anti-UCP1 antibody (<a href="/products/HA751580" style="font-weight: bold;text-decoration: underline;">HA751580</a>) at 1/2,000 dilution.<br /><br />Lane 1: Mouse brown adipose tissue lysate<br />Lane 2: Rat brown adipose tissue lysate<br />Lane 3: Rat white adipose tissue lysate (negative)<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 33 kDa<br />Observed band size: 33 kDa<br /><br />Exposure time: 3 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751580" style="font-weight: bold;text-decoration: underline;">HA751580</a>) at 1/2,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of UCP1 on different lysates with Rabbit anti-UCP1 antibody (HA751580) at 1/2,000 dilution.

    Lane 1: Mouse brown adipose tissue lysate
    Lane 2: Rat brown adipose tissue lysate
    Lane 3: Rat white adipose tissue lysate (negative)

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 33 kDa
    Observed band size: 33 kDa

    Exposure time: 3 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751580) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: brown adipose<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: brown adipose

    Sample: Frozen section

    Antibody concentration: 1/500

    Antigen retrieval: Not required

  • Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue with Rabbit anti-UCP1 antibody (<a href="/products/HA751580" style="font-weight: bold;text-decoration: underline;">HA751580</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751580" style="font-weight: bold;text-decoration: underline;">HA751580</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue with Rabbit anti-UCP1 antibody (HA751580) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751580) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunohistochemical analysis of paraffin-embedded mouse white adipose tissue (negative) with Rabbit anti-UCP1 antibody (<a href="/products/HA751580" style="font-weight: bold;text-decoration: underline;">HA751580</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751580" style="font-weight: bold;text-decoration: underline;">HA751580</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    ☑ Relative expression (RE)

    Immunohistochemical analysis of paraffin-embedded mouse white adipose tissue (negative) with Rabbit anti-UCP1 antibody (HA751580) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751580) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat brown adipose tissue with Rabbit anti-UCP1 antibody (<a href="/products/HA751580" style="font-weight: bold;text-decoration: underline;">HA751580</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751580" style="font-weight: bold;text-decoration: underline;">HA751580</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat brown adipose tissue with Rabbit anti-UCP1 antibody (HA751580) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751580) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunohistochemical analysis of paraffin-embedded rat white adipose tissue (negative) with Rabbit anti-UCP1 antibody (<a href="/products/HA751580" style="font-weight: bold;text-decoration: underline;">HA751580</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751580" style="font-weight: bold;text-decoration: underline;">HA751580</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    ☑ Relative expression (RE)

    Immunohistochemical analysis of paraffin-embedded rat white adipose tissue (negative) with Rabbit anti-UCP1 antibody (HA751580) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751580) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • UCP1 was immunoprecipitated from 0.2 mg mouse brown adipose tissue lysate with <a href="/products/HA751580" style="font-weight: bold;text-decoration: underline;">HA751580</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA751580" style="font-weight: bold;text-decoration: underline;">HA751580</a> at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: mouse brown adipose tissue lysate (input)<br />Lane 2: <a href="/products/HA751580" style="font-weight: bold;text-decoration: underline;">HA751580</a> IP in mouse brown adipose tissue lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA751580" style="font-weight: bold;text-decoration: underline;">HA751580</a> in mouse brown adipose tissue lysate<br /><br />Blocking/Dilution buffer: primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>)<br />Exposure time: 3 seconds; ECL: <a href="/products/K1801" style="font-weight: bold;text-decoration: underline;">K1801</a>

    UCP1 was immunoprecipitated from 0.2 mg mouse brown adipose tissue lysate with HA751580 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751580 at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: mouse brown adipose tissue lysate (input)
    Lane 2: HA751580 IP in mouse brown adipose tissue lysate
    Lane 3: Rabbit IgG instead of HA751580 in mouse brown adipose tissue lysate

    Blocking/Dilution buffer: primary antibody dilution (K1803)
    Exposure time: 3 seconds; ECL: K1801

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