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SCNN1G Recombinant Rabbit Monoclonal Antibody [PSH16-29] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751597

SCNN1G Recombinant Rabbit Monoclonal Antibody [PSH16-29] - BSA and Azide free

Application

  • WB

  • IF-Cell

Reactivity

  • Human

  • Mouse

  • Rat

  • Green monkey

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

SCNN1G Recombinant Rabbit Monoclonal Antibody [PSH16-29] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human SCNN1G aa 77-541.

Species Reactivity

Human, Mouse, Rat, Green monkey

Validated Applications

WB, IF-Cell

Target Molecular Weight

Predicted band size: 74 kDa

Positive Control

A549 cell lysate, A431 cell lysate, 293T cell lysate, COS-1 cell lysate, C2C12 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, C6 cell lysate, Mouse kidney tissue lysate, Mouse lung tissue lysate, Mouse colon tissue lysate, Rat kidney tissue lysate, Rat lung tissue lysate, Rat colon tissue lysate, 293T, PC-12.

Conjugation

unconjugated

Clone Number

PSH16-29

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at 2-8℃. Avoid freeze.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IF-Cell

  • 1:100-1:500

Target

Function

This is one of the three pore-forming subunits of the heterotrimeric epithelial sodium channel (ENaC), a critical regulator of sodium balance and fluid homeostasis. ENaC operates in epithelial tissues, where it mediates the electrodiffusion of sodium ions from extracellular fluid through the apical membrane of cells, with water following osmotically. It plays a key role in maintaining sodium homeostasis through electrogenic sodium reabsorption in the kidneys . Additionally, ENaC is essential for airway surface liquid homeostasis, which is crucial for proper mucus clearance.

Background References

1. Noreng S, Bharadwaj A, Posert R, Yoshioka C, Baconguis I. Structure of the human epithelial sodium channel by cryo-electron microscopy. Elife. 2018 Sep 25;7:e39340. doi: 10.7554/eLife.39340. PMID: 30251954; PMCID: PMC6197857.

2. Hansson JH, Nelson-Williams C, Suzuki H, Schild L, Shimkets R, Lu Y, Canessa C, Iwasaki T, Rossier B, Lifton RP. Hypertension caused by a truncated epithelial sodium channel gamma subunit: genetic heterogeneity of Liddle syndrome. Nat Genet. 1995 Sep;11(1):76-82. doi: 10.1038/ng0995-76. PMID: 7550319.

Subcellular Location

Apical cell membrane.

UNIPROT

SwissProt: P51170 Human

SwissProt: Q9WU39 Mouse

SwissProt: P37091 Rat

Synonyms

Epithelial sodium channel subunit gamma

ENaC subunit gamma

ENaCG

Epithelial Na(+) channel subunit gamma

Gamma-ENaC

Amiloride-sensitive sodium channel subunit gamma

Gamma-NaCH

Nonvoltage-gated sodium channel 1 subunit gamma

SCNEG

Sodium channel epithelial 1 subunit gamma

Expand

Images

  • Western blot analysis of SCNN1G on different lysates with Rabbit anti-SCNN1G antibody (<a href="/products/HA751597" style="font-weight: bold;text-decoration: underline;">HA751597</a>) at 1/5,000 dilution.<br /><br />Lane 1: A549 cell lysate (20 µg/Lane)<br />Lane 2: A431 cell lysate (20 µg/Lane)<br />Lane 3: 293T cell lysate (20 µg/Lane) <br />Lane 4: COS-1 cell lysate (20 µg/Lane) <br />Lane 5: C2C12 cell lysate (20 µg/Lane) <br />Lane 6: NIH/3T3 cell lysate (20 µg/Lane) <br />Lane 7: PC-12 cell lysate (20 µg/Lane) <br />Lane 8: C6 cell lysate (20 µg/Lane) <br />Lane 9: Mouse kidney tissue lysate (40 µg/Lane)<br />Lane 10: Mouse lung tissue lysate (40 µg/Lane)<br />Lane 11: Mouse colon tissue lysate (40 µg/Lane)<br />Lane 12: Rat kidney tissue lysate (40 µg/Lane)<br />Lane 13: Rat lung tissue lysate (40 µg/Lane)<br />Lane 14: Rat colon tissue lysate (40 µg/Lane) <br /><br />Predicted band size: 74 kDa<br />Observed band size: 74 kDa<br /><br />Exposure time: 16 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751597" style="font-weight: bold;text-decoration: underline;">HA751597</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of SCNN1G on different lysates with Rabbit anti-SCNN1G antibody (HA751597) at 1/5,000 dilution.

    Lane 1: A549 cell lysate (20 µg/Lane)
    Lane 2: A431 cell lysate (20 µg/Lane)
    Lane 3: 293T cell lysate (20 µg/Lane)
    Lane 4: COS-1 cell lysate (20 µg/Lane)
    Lane 5: C2C12 cell lysate (20 µg/Lane)
    Lane 6: NIH/3T3 cell lysate (20 µg/Lane)
    Lane 7: PC-12 cell lysate (20 µg/Lane)
    Lane 8: C6 cell lysate (20 µg/Lane)
    Lane 9: Mouse kidney tissue lysate (40 µg/Lane)
    Lane 10: Mouse lung tissue lysate (40 µg/Lane)
    Lane 11: Mouse colon tissue lysate (40 µg/Lane)
    Lane 12: Rat kidney tissue lysate (40 µg/Lane)
    Lane 13: Rat lung tissue lysate (40 µg/Lane)
    Lane 14: Rat colon tissue lysate (40 µg/Lane)

    Predicted band size: 74 kDa
    Observed band size: 74 kDa

    Exposure time: 16 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751597) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of 293T cells labeling SCNN1G with Rabbit anti-SCNN1G antibody (<a href="/products/HA751597" style="font-weight: bold;text-decoration: underline;">HA751597</a>) at 1/100 dilution.<br />Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SCNN1G antibody (<a href="/products/HA751597" style="font-weight: bold;text-decoration: underline;">HA751597</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br />Beta tubulin (<a href="/products/HA601187" style="font-weight: bold;text-decoration: underline;">HA601187</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of 293T cells labeling SCNN1G with Rabbit anti-SCNN1G antibody (HA751597) at 1/100 dilution.
    Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SCNN1G antibody (HA751597) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
    Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • Immunocytochemistry analysis of PC-12 cells labeling SCNN1G with Rabbit anti-SCNN1G antibody (<a href="/products/HA751597" style="font-weight: bold;text-decoration: underline;">HA751597</a>) at 1/500 dilution.<br />Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SCNN1G antibody (<a href="/products/HA751597" style="font-weight: bold;text-decoration: underline;">HA751597</a>) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br />Beta tubulin (<a href="/products/HA601187" style="font-weight: bold;text-decoration: underline;">HA601187</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of PC-12 cells labeling SCNN1G with Rabbit anti-SCNN1G antibody (HA751597) at 1/500 dilution.
    Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SCNN1G antibody (HA751597) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
    Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

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