SCNN1G Recombinant Rabbit Monoclonal Antibody [PSH16-29]
Catalog# HA723827
SCNN1G Recombinant Rabbit Monoclonal Antibody [PSH16-29]
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WB
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IF-Cell
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Human
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Mouse
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Rat
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Green monkey
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unconjugated
Overview
Product Name
SCNN1G Recombinant Rabbit Monoclonal Antibody [PSH16-29]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human SCNN1G aa 77-541.
Species Reactivity
Human, Mouse, Rat, Green monkey
Validated Applications
WB, IF-Cell
Target Molecular Weight
Predicted band size: 74 kDa
Positive Control
A549 cell lysate, A431 cell lysate, 293T cell lysate, COS-1 cell lysate, C2C12 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, C6 cell lysate, Mouse kidney tissue lysate, Mouse lung tissue lysate, Mouse colon tissue lysate, Rat kidney tissue lysate, Rat lung tissue lysate, Rat colon tissue lysate, 293T, PC-12.
Conjugation
unconjugated
Clone Number
PSH16-29
Reactivity Data
| WB | IF-Cell | |
|---|---|---|
| Human |
|
|
| Mouse |
|
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| Rat |
|
|
| Green Monkey |
|
Product Features
Form
Liquid
Concentration
1 mg/ml(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:5,000
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IF-Cell
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1:100-1:500
Target
Function
This is one of the three pore-forming subunits of the heterotrimeric epithelial sodium channel (ENaC), a critical regulator of sodium balance and fluid homeostasis. ENaC operates in epithelial tissues, where it mediates the electrodiffusion of sodium ions from extracellular fluid through the apical membrane of cells, with water following osmotically. It plays a key role in maintaining sodium homeostasis through electrogenic sodium reabsorption in the kidneys . Additionally, ENaC is essential for airway surface liquid homeostasis, which is crucial for proper mucus clearance.
Background References
1. Noreng S, Bharadwaj A, Posert R, Yoshioka C, Baconguis I. Structure of the human epithelial sodium channel by cryo-electron microscopy. Elife. 2018 Sep 25;7:e39340. doi: 10.7554/eLife.39340. PMID: 30251954; PMCID: PMC6197857.
2. Hansson JH, Nelson-Williams C, Suzuki H, Schild L, Shimkets R, Lu Y, Canessa C, Iwasaki T, Rossier B, Lifton RP. Hypertension caused by a truncated epithelial sodium channel gamma subunit: genetic heterogeneity of Liddle syndrome. Nat Genet. 1995 Sep;11(1):76-82. doi: 10.1038/ng0995-76. PMID: 7550319.
Subcellular Location
Apical cell membrane.
Synonyms
Epithelial sodium channel subunit gamma
ENaC subunit gamma
ENaCG
Epithelial Na(+) channel subunit gamma
Gamma-ENaC
Amiloride-sensitive sodium channel subunit gamma
Gamma-NaCH
Nonvoltage-gated sodium channel 1 subunit gamma
SCNEG
Sodium channel epithelial 1 subunit gamma
ExpandImages
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Western blot analysis of SCNN1G on different lysates with Rabbit anti-SCNN1G antibody (HA723827) at 1/5,000 dilution.
Lane 1: A549 cell lysate (20 µg/Lane)
Lane 2: A431 cell lysate (20 µg/Lane)
Lane 3: 293T cell lysate (20 µg/Lane)
Lane 4: COS-1 cell lysate (20 µg/Lane)
Lane 5: C2C12 cell lysate (20 µg/Lane)
Lane 6: NIH/3T3 cell lysate (20 µg/Lane)
Lane 7: PC-12 cell lysate (20 µg/Lane)
Lane 8: C6 cell lysate (20 µg/Lane)
Lane 9: Mouse kidney tissue lysate (40 µg/Lane)
Lane 10: Mouse lung tissue lysate (40 µg/Lane)
Lane 11: Mouse colon tissue lysate (40 µg/Lane)
Lane 12: Rat kidney tissue lysate (40 µg/Lane)
Lane 13: Rat lung tissue lysate (40 µg/Lane)
Lane 14: Rat colon tissue lysate (40 µg/Lane)
Predicted band size: 74 kDa
Observed band size: 74 kDa
Exposure time: 16 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723827) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of 293T cells labeling SCNN1G with Rabbit anti-SCNN1G antibody (HA723827) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SCNN1G antibody (HA723827) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunocytochemistry analysis of PC-12 cells labeling SCNN1G with Rabbit anti-SCNN1G antibody (HA723827) at 1/500 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SCNN1G antibody (HA723827) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"