TMS1 / ASC Recombinant Rabbit Monoclonal Antibody [PSH17-34] - BSA and Azide free
Catalog# HA751622
TMS1 / ASC Recombinant Rabbit Monoclonal Antibody [PSH17-34] - BSA and Azide free
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WB
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IHC-Fr
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IHC-P
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Human
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Mouse
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HA723917
含抗保成分
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unconjugated
Overview
Product Name
TMS1 / ASC Recombinant Rabbit Monoclonal Antibody [PSH17-34] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within mouse TMS1 / ASC aa 1-193.
Species Reactivity
Human, Mouse
Validated Applications
WB, IHC-Fr, IHC-P
Molecular Weight
Predicted band size: 21 kDa
Positive Control
Mouse spleen tissue lysate, Mouse colon tissue lysate, HL-60 cell lysate, THP-1 cell lysate, U-937 cell lysate, human spleen tissue, mouse spleen tissue.
Conjugation
unconjugated
Clone Number
PSH17-34
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:5,000
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IHC-Fr
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1:500
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IHC-P
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1:200-1:1,000
Target
Function
PYCARD, often referred to as ASC (Apoptosis-associated speck-like protein containing a CARD), is a protein that in humans is encoded by the PYCARD gene. It is localized mainly in the nucleus of monocytes and macrophages. In case of pathogen infection, however, it relocalizes rapidly to the cytoplasm, perinuclear space, endoplasmic reticulum and mitochondria and it is a key adaptor protein in activation of the inflammasome.
Background References
1. Jiang H et al. Pristimerin suppresses AIM2 inflammasome by modulating AIM2-PYCARD/ASC stability via selective autophagy to alleviate tendinopathy. Autophagy. 2024 Jan
2. Suganuma Y et al. Expression of a PYCARD/ASC variant lacking exon 2 in Japanese patients with palindromic rheumatism increases interleukin-1beta secretion. Asian Pac J Allergy Immunol. 2022 Mar
Subcellular Location
Cytoplasm, Inflammasome, Endoplasmic reticulum, Mitochondrion, Nucleus.
Synonyms
Apoptosis associated speck like protein containing a CARD antibody
Apoptosis-associated speck-like protein containing a CARD antibody
ASC antibody
CARD 5 antibody
CARD5 antibody
Caspase recruitment domain containing protein 5 antibody
Caspase recruitment domain protein 5 antibody
Caspase recruitment domain-containing protein 5 antibody
hASC antibody
MGC10332 antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of TMS1 / ASC on different lysates with Rabbit anti-TMS1 / ASC antibody (HA751622) at 1/5,000 dilution.
Lane 1: Mouse spleen tissue lysate
Lane 2: Mouse colon tissue lysate
Lane 3: Mouse skeletal muscle tissue lysate (low expression)
Lysates/proteins at 30 µg/Lane.
Predicted band size: 21 kDa
Observed band size: 25 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751622) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of TMS1 / ASC on different lysates with Rabbit anti-TMS1 / ASC antibody (HA751622) at 1/5,000 dilution.
Lane 1: HL-60 cell lysate
Lane 2: THP-1 cell lysate
Lane 3: U-937 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 21 kDa
Observed band size: 25 kDa
Exposure time: 3 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751622) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Application: IHC-Fr
Species: Mouse
Site: colon
Sample: Frozen section
Antibody concentration: 1/500
Antigen retrieval: Not required -
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-TMS1 / ASC antibody (HA751622) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751622) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-TMS1 / ASC antibody (HA751622) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751622) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue (low expression) with Rabbit anti-TMS1 / ASC antibody (HA751622) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751622) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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