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TMS1 / ASC Recombinant Rabbit Monoclonal Antibody [PSH17-34]

Usd: 385

Specification

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Catalog# HA723917

TMS1 / ASC Recombinant Rabbit Monoclonal Antibody [PSH17-34]

Application

  • WB

  • IHC-Fr

  • IHC-P

Reactivity

  • Human

  • Mouse

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

TMS1 / ASC Recombinant Rabbit Monoclonal Antibody [PSH17-34]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within mouse TMS1 / ASC aa 1-193.

Species Reactivity

Human, Mouse

Validated Applications

WB, IHC-Fr, IHC-P

Molecular Weight

Predicted band size: 21 kDa

Positive Control

Mouse spleen tissue lysate, Mouse colon tissue lysate, HL-60 cell lysate, THP-1 cell lysate, U-937 cell lysate, human spleen tissue, mouse spleen tissue.

Conjugation

unconjugated

Clone Number

PSH17-34

Reactivity Data

WB IHC-Fr IHC-P
Human
Tested Tested
Tested Tested
Mouse
Tested Tested
Tested Tested
Tested Tested
Rat
Not Recommended

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IHC-Fr

  • 1:500

  • IHC-P

  • 1:200-1:1,000

Target

Function

PYCARD, often referred to as ASC (Apoptosis-associated speck-like protein containing a CARD), is a protein that in humans is encoded by the PYCARD gene. It is localized mainly in the nucleus of monocytes and macrophages. In case of pathogen infection, however, it relocalizes rapidly to the cytoplasm, perinuclear space, endoplasmic reticulum and mitochondria and it is a key adaptor protein in activation of the inflammasome.

Background References

1. Jiang H et al. Pristimerin suppresses AIM2 inflammasome by modulating AIM2-PYCARD/ASC stability via selective autophagy to alleviate tendinopathy. Autophagy. 2024 Jan

2. Suganuma Y et al. Expression of a PYCARD/ASC variant lacking exon 2 in Japanese patients with palindromic rheumatism increases interleukin-1beta secretion. Asian Pac J Allergy Immunol. 2022 Mar

Subcellular Location

Cytoplasm, Inflammasome, Endoplasmic reticulum, Mitochondrion, Nucleus.

UNIPROT

SwissProt: Q9ULZ3 Human

SwissProt: Q9EPB4 Mouse

Synonyms

Apoptosis associated speck like protein containing a CARD antibody

Apoptosis-associated speck-like protein containing a CARD antibody

ASC antibody

CARD 5 antibody

CARD5 antibody

Caspase recruitment domain containing protein 5 antibody

Caspase recruitment domain protein 5 antibody

Caspase recruitment domain-containing protein 5 antibody

hASC antibody

MGC10332 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of TMS1 / ASC on different lysates with Rabbit anti-TMS1 / ASC antibody (<a href="/products/HA723917" style="font-weight: bold;text-decoration: underline;">HA723917</a>) at 1/5,000 dilution.<br /><br />Lane 1: Mouse spleen tissue lysate<br />Lane 2: Mouse colon tissue lysate<br />Lane 3: Mouse skeletal muscle tissue lysate (low expression)<br /><br />Lysates/proteins at 30 µg/Lane.<br /><br />Predicted band size: 21 kDa<br />Observed band size: 25 kDa<br /><br />Exposure time: 20 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723917" style="font-weight: bold;text-decoration: underline;">HA723917</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of TMS1 / ASC on different lysates with Rabbit anti-TMS1 / ASC antibody (HA723917) at 1/5,000 dilution.

    Lane 1: Mouse spleen tissue lysate
    Lane 2: Mouse colon tissue lysate
    Lane 3: Mouse skeletal muscle tissue lysate (low expression)

    Lysates/proteins at 30 µg/Lane.

    Predicted band size: 21 kDa
    Observed band size: 25 kDa

    Exposure time: 20 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723917) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of TMS1 / ASC on different lysates with Rabbit anti-TMS1 / ASC antibody (<a href="/products/HA723917" style="font-weight: bold;text-decoration: underline;">HA723917</a>) at 1/5,000 dilution.<br /><br />Lane 1: HL-60 cell lysate<br />Lane 2: THP-1 cell lysate<br />Lane 3: U-937 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 21 kDa<br />Observed band size: 25 kDa<br /><br />Exposure time: 3 minutes; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723917" style="font-weight: bold;text-decoration: underline;">HA723917</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of TMS1 / ASC on different lysates with Rabbit anti-TMS1 / ASC antibody (HA723917) at 1/5,000 dilution.

    Lane 1: HL-60 cell lysate
    Lane 2: THP-1 cell lysate
    Lane 3: U-937 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 21 kDa
    Observed band size: 25 kDa

    Exposure time: 3 minutes; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723917) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: colon<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: colon

    Sample: Frozen section

    Antibody concentration: 1/500

    Antigen retrieval: Not required

  • Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-TMS1 / ASC antibody (<a href="/products/HA723917" style="font-weight: bold;text-decoration: underline;">HA723917</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723917" style="font-weight: bold;text-decoration: underline;">HA723917</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-TMS1 / ASC antibody (HA723917) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723917) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-TMS1 / ASC antibody (<a href="/products/HA723917" style="font-weight: bold;text-decoration: underline;">HA723917</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723917" style="font-weight: bold;text-decoration: underline;">HA723917</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-TMS1 / ASC antibody (HA723917) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723917) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue (low expression) with Rabbit anti-TMS1 / ASC antibody (<a href="/products/HA723917" style="font-weight: bold;text-decoration: underline;">HA723917</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723917" style="font-weight: bold;text-decoration: underline;">HA723917</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    ☑ Relative expression (RE)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue (low expression) with Rabbit anti-TMS1 / ASC antibody (HA723917) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723917) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

Products with the same target and pathway

metafield image

TMS1 / ASC Recombinant Rabbit Monoclonal Antibody [PSH17-34] - BSA and Azide free

Application: WB,IHC-Fr,IHC-P

Reactivity: Human,Mouse

Conjugate: unconjugated