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PDE1B Recombinant Rabbit Monoclonal Antibody [PSH17-59] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751633

PDE1B Recombinant Rabbit Monoclonal Antibody [PSH17-59] - BSA and Azide free

Application

  • WB

  • IHC-P

  • IHC-Fr

Reactivity

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

PDE1B Recombinant Rabbit Monoclonal Antibody [PSH17-59] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human PDE1B aa 401-536.

Species Reactivity

Mouse, Rat

Validated Applications

WB, IHC-P, IHC-Fr

Molecular Weight

Predicted band size: 61 kDa

Positive Control

Mouse brain tissue lysate, Rat brain tissue lysate, Rat cerebellum tissue lysate, Rat hippocampus tissue lysate, mouse cerebrum tissue, mouse corpus striatum tissue, rat cerebellum tissue.

Conjugation

unconjugated

Clone Number

PSH17-59

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IHC-P

  • 1:1,000

  • IHC-Fr

  • 1:500

Target

Function

Cyclic nucleotide phosphodiesterase with a dual specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a preference for cGMP as a substrate.

Background References

1. Chen Q et al. PDE1B, a potential biomarker associated with tumor microenvironment and clinical prognostic significance in osteosarcoma. Sci Rep. 2024 Jun

2. Al-Nema M et al. Designing of 2,3-dihydrobenzofuran derivatives as inhibitors of PDE1B using pharmacophore screening, ensemble docking and molecular dynamics approach. Comput Biol Med. 2023 Jun

Subcellular Location

Cytoplasm, cytosol.

UNIPROT

SwissProt: Q01065 Mouse

SwissProt: Q01066 Rat

Synonyms

5''-cyclic nucleotide phosphodiesterase 1B antibody

63 kDa Cam PDE antibody

63 kDa Cam-PDE antibody

Calcium/calmodulin dependent 3' 5' cyclic nucleotide Phosphodiesterase 1B antibody

Calcium/calmodulin stimulated cyclic nucleotide phosphodiesterase antibody

Calcium/calmodulin-dependent 3'' antibody

Calmodulin stimulated phosphodiesterase PDE1B1 antibody

Cam PDE 1B antibody

Cam PDE1B antibody

Cam-PDE 1B antibody

Expand

Images

  • Western blot analysis of PDE1B on different lysates with Rabbit anti-PDE1B antibody (<a href="/products/HA751633" style="font-weight: bold;text-decoration: underline;">HA751633</a>) at 1/5,000 dilution.<br /><br />Lane 1: Mouse brain tissue lysate (10 µg/Lane)<br />Lane 2: Rat brain tissue lysate (10 µg/Lane)<br />Lane 3: Rat cerebellum tissue lysate (30 µg/Lane)<br />Lane 4: Rat hippocampus tissue lysate (20 µg/Lane)<br /><br />Predicted band size: 61 kDa<br />Observed band size: 61 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751633" style="font-weight: bold;text-decoration: underline;">HA751633</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of PDE1B on different lysates with Rabbit anti-PDE1B antibody (HA751633) at 1/5,000 dilution.

    Lane 1: Mouse brain tissue lysate (10 µg/Lane)
    Lane 2: Rat brain tissue lysate (10 µg/Lane)
    Lane 3: Rat cerebellum tissue lysate (30 µg/Lane)
    Lane 4: Rat hippocampus tissue lysate (20 µg/Lane)

    Predicted band size: 61 kDa
    Observed band size: 61 kDa

    Exposure time: 10 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751633) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue with Rabbit anti-PDE1B antibody (<a href="/products/HA751633" style="font-weight: bold;text-decoration: underline;">HA751633</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751633" style="font-weight: bold;text-decoration: underline;">HA751633</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue with Rabbit anti-PDE1B antibody (HA751633) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751633) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse corpus striatum tissue with Rabbit anti-PDE1B antibody (<a href="/products/HA751633" style="font-weight: bold;text-decoration: underline;">HA751633</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751633" style="font-weight: bold;text-decoration: underline;">HA751633</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse corpus striatum tissue with Rabbit anti-PDE1B antibody (HA751633) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751633) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-PDE1B antibody (<a href="/products/HA751633" style="font-weight: bold;text-decoration: underline;">HA751633</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751633" style="font-weight: bold;text-decoration: underline;">HA751633</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-PDE1B antibody (HA751633) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751633) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: brain (striatum)<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: brain (striatum)

    Sample: Frozen section

    Antibody concentration: 1/500

    Antigen retrieval: Not required

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"